Recombinant Anti-Aminoacylase 1 Antibody, Rabbit Monoclonal
EliteRmab®, manufactured by recombinant technology
Cat: 10549-R006
Recombinant Anti-Aminoacylase 1 Antibody, Rabbit Monoclonal General Information
Product name
Recombinant Anti-Aminoacylase 1 Antibody, Rabbit Monoclonal
Validated applications
FCM,ICC/IF
Species reactivity
Reacts with: Human
Specificity
Human Aminoacylase 1
Immunogen
Recombinant Human ACY1 / Aminoacylase-1 protein (Catalog#10549-H08B)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human ACY1 / Aminoacylase-1 (rh ACY1 / Aminoacylase-1; Catalog#10549-H08B; NP_000657.1; Met1-Ser408).
Source
Monoclonal Rabbit IgG Clone #006
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Recombinant Anti-Aminoacylase 1 Antibody, Rabbit Monoclonal Validated Applications
| Application | Dilution |
|---|---|
| ICC-IF | 1:20-1:100 |
| FCM | 1:25-1:100 |
Please Note: Optimal concentrations/dilutions should be determined by the end user.
Recombinant Anti-Aminoacylase 1 Antibody, Rabbit Monoclonal Images
-SKBR3-C(L).png)
Immunofluorescence staining of Human ACY1 in Hela or SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human ACY1 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI (blue). Positive staining was localized to cytoplasm.
Flow cytometric analysis of Human ACY1 expression on HepG2 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human ACY1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Citation List
Powered by BiozRecombinant Anti-Aminoacylase 1 Antibody, Rabbit Monoclonal: Synonyms
Anti-ACY-1 Antibody; Anti-ACY1D Antibody; Anti-HEL-S-5 Antibody
Aminoacylase 1 Background Information
Aminoacylase 1 (ACY1), a metalloenzyme that removes amide-linked ACY1 groups from amino acids and may play a role in regulating responses to oxidative stress. Both the C-terminal fragment found in the two-hybrid screen and full-length ACY1 co-immunoprecipitate with SphK1. Though both C-terminal and full-length proteins slightly reduce SphK1 activity measured in vitro, the C-terminal fragment inhibits while full-length ACY1 potentiates the effects of SphK1 on proliferation and apoptosis. It suggested that ACY1 physically interacts with SphK1 and may influence its physiological functions. As a homodimeric zinc-binding enzyme, Aminoacylase 1 catalyzes the hydrolysis of N alpha-acylated amino acids. Deficiency of Aminoacylase 1 due to mutations in the Aminoacylase 1 (ACY1) gene follows an autosomal-recessive trait of inheritance and is characterized by accumulation of N-acetyl amino acids in the urine.
Full Name
aminoacylase 1
References
- Sommer A, et al. (2011) The molecular basis of aminoacylase 1 deficiency. Biochim Biophys Acta. 1812(6): 685-90.
- Maceyka M, et al. (2004) Aminoacylase 1 is a sphingosine kinase 1-interacting protein. FEBS Lett. 568(1-3): 30-4.
- Cook RM, et al.(1993) Human aminoacylase-1. Cloning, sequence, and expression analysis of a chromosome 3p21 gene inactivated in small cell lung cancer. J Biol Chem. 268(23): 17010-7.
Recombinant Antibody Production Service
High-throughput Recombinant Antibody Production
