Anti-GFP Tag Antibody, Rabbit Polyclonal

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Anti-GFP Tag Antibody, Rabbit Polyclonal General Information

Product name
Anti-GFP Tag Antibody, Rabbit Polyclonal
Validated applications
ELISA
Species reactivity
Reacts with: Aequorea victoria
Specificity
Aequorea victoria GFP Tag
Immunogen
Recombinant Aequorea victoria GFP protein (Catalog#13105-S07E)
Preparation
Produced in rabbits immunized with purified, recombinant Aequorea victoria GFP (Catalog#13105-S07E; AAB65663; Ser2-Lys238). GFP specific IgG was purified by Aequorea victoria GFP affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-GFP Tag Antibody, Rabbit Polyclonal Validated Applications

Application Dilution
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

GFP Tag Background Information

Green fluorescent protein (GFP tag) is a ~27 kDa protein consisting of 238 amino acids derived from jellyfish Aequorea Victoria. GFP exhibits bright green fluorescent light when exposed to blue light. It has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms. Anti GFP-tag antibodies are often used in biological research and related fields. GFP tag antibodies are also often been used to study the expression of proteins and the interaction between proteins and proteins.
References
  • Zhao X, et al. (2013) Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem 2013 581093.
  • Monti M, et al. (2007) Functional proteomics: Protein-protein interactions in vivo. Ital J Biochem 56 (4): 310-314.
  • Brothers SP, et al. (2003) Unexpected effects of epitope and chimeric tags on gonadotropin-releasing hormone receptors: Implications for understanding the molecular etiology of hypogonadotropic hypogonadism. J Clin Endocrinol Metab 88 (12): 6107-6112.

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