SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody, Mouse MAb

RRID Number: AB_2827977
COVID-19 Nucleocapsid Research.
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SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody, Mouse MAb General Information

Product name
SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody, Mouse MAb
Validated applications
WB,ELISA
Application notes
IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
SARS Coronavirus Nucleocapsid
Has cross-reactivity in ELISA and WB with
SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (Cat# 40588-V08B)
No cross-reactivity in ELISA with
MERS-CoV Nucleoprotein protein (40068-V08B)
HCoV-229E Nucleoprotein protein (40640-V07E)
HCoV-NL63 Nucleoprotein protein (40641-V07E)
HCoV-HKU1(isolate N5) Nucleoprotein protein (40642-V07E)
HCoV-OC43 Nucleoprotein (40643-V07E)
Immunogen
Recombinant SARS-CoV Nucleoprotein / NP Protein (Catalog#40143-V08B)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant SARS-CoV Nucleoprotein / NP (Catalog#40143-V08B; NP_828858.1; Met1-Ala422). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #05
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody, Mouse MAb Validated Applications

Application Dilution
WB 1:1000-1:5000
ELISA 1:1000-1:2000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody, Mouse MAb Images

Anti-SARS-NP mouse monoclonal antibody at 1:1000 dilution.

Lane A: SARS-CoV NP Protein (Cat#40143-V08B) (30ng)

Lane B: SARS-CoV NP Protein (Cat#40143-V08B) (5ng)

Lane C: SARS-CoV-2 (2019-nCoV) NP Protein (Cat#40588-V08B) (30ng)

Lane D: SARS-CoV-2 (2019-nCoV) NP Protein (Cat#40588-V08B) (5ng)

Secondary

Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody, Mouse MAb: Synonyms

Anti-coronavirus NP Antibody; Anti-coronavirus Nucleocapsid Antibody; Anti-coronavirus Nucleoprotein Antibody; Anti-cov np Antibody; Anti-ncov NP Antibody; Anti-novel coronavirus NP Antibody; Anti-novel coronavirus Nucleocapsid Antibody; Anti-novel coronavirus Nucleoprotein Antibody; Anti-NP Antibody; Anti-Nucleocapsid Antibody; Anti-Nucleoprotein Antibody

Coronavirus Nucleocapsid Background Information

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
References
    1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
    2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
    3. Li W. et al., 2005, Science. 309 (5742): 1864-8.
  • Molecular Detection of SARS-CoV-2 in Formalin Fixed Paraffin Embedded Specimens
    Author
    Zeng, X;Liu, J;Babka, A;Kearney, B;Radoshitzky, S;
    Year
    2020
    Journal
    bioRxiv
  • Critical Role of Type III Interferon in Controlling SARS-CoV-2 Infection in Human Intestinal Epithelial Cells
    Author
    Stanifer, ML;Kee, C;Cortese, M;Zumaran, CM;Triana, S;Mukenhirn, M;Kraeusslich, HG;Alexandrov, T;Bartenschlager, R;Boulant, S;
    Year
    2020
    Journal
    Cell Rep
    Application
    IF; WB;
  • SARS-CoV-2 infection and replication in human fetal and pediatric gastric organoids
    Author
    Giobbe, G;Bonfante, F;Zambaiti, E;Gagliano, O;Jones, B;Luni, C;Laterza, C;Perin, S;Stuart, H;Pagliari, M;Bortolami, A;Mazzetto, E;Manfredi, A;Colantuono, C;Di Filippo, L;Pellegata, A;Li, V;Eaton, S;Thapar, N;Cacchiarelli, D;Elvassore, N;De Coppi, P;
    Year
    2020
    Journal
    bioRxiv
    Application
    Elisa
  • SARS-CoV-2 induces activation and diversification of human plasmacytoid pre-dendritic cells
    Author
    Onodi, F;Bonnet-Madin, L;Karpf, L;Meertens, L;Poirot, J;Legoff, J;Delaugerre, C;Amara, A;Soumelis, V;
    Year
    2020
    Journal
    bioRxiv
    Application
    FCM
  • Human angiotensin-converting enzyme 2 transgenic mice infected with SARS-CoV-2 develop severe and fatal respiratory disease
    Author
    Golden, J;Cline, C;Zeng, X;Garrison, A;Carey, B;Mucker, E;White, L;Shamblin, J;Brocato, R;Liu, J;Babka, A;Rauch, H;Smith, J;Hollidge, B;Fitzpatrick, C;Badger, C;Hooper, J;
    Year
    2020
    Journal
    bioRxiv
  • Structures, conformations and distributions of SARS-CoV-2 spike protein trimers on intact virions
    Author
    Ke, Z;Oton, J;Qu, K;Cortese, M;Zila, V;McKeane, L;Nakane, T;Zivanov, J;Neufeldt, C;Lu, J;Peukes, J;Xiong, X;Kräusslich, H;Scheres, S;Bartenschlager, R;Briggs, J;
    Year
    2020
    Journal
    bioRxiv
    Application
    WB

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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