Anti-GST Tag Antibody

Anti-GST Tag Antibody

The GST (Glutathione S-transferase) tag is a well-established affinity tag based on the strong affinity of GST for immobilized glutathione. The GST tag is best suitable for use in prokaryotic expression because GSTs are a family of multifunctional cytosolic proteins that are present in eukaryotic organisms but generally not found in bacteria. The GST tag contains 220 amino acids, and has a molecular weight of 26 KDa. Anti-GST Tag antibodies can be used for detection and purification of GST-tagged target proteins without a protein-specific antibody or probe.

Sino Biological anti-GST Tag antibodies can reliably detect recombinant GST-tagged proteins, and every lot of our anti-GST Tag antibodies are fully validated, including Western Blotting (WB), ELISA and Immunoprecipitation (IP) applications.

Anti-GST Tag Antibody Product List

Product description Application Catalog# (PDF) Size Price Select
GST Tag Antibody, Rabbit PAb, Antigen Affinity Purified ELISA 11213-RP04 50 µL  
11213-RP04 100 µL  
11213-RP04 500 µL  
11213-RP04 1ml  
11213-RP04 5ml  
GST Tag Antibody, Mouse MAb WB IP 11213-MM01 50 µL  
11213-MM01 100 µL  
11213-MM01 500 µL  
11213-MM01 1ml  
11213-MM01 5ml  
GST Tag Antibody, Mouse MAb ELISA 11213-MM02 50 µL  
11213-MM02 100 µL  
11213-MM02 500 µL  
11213-MM02 1ml  
11213-MM02 5ml  
GST Tag Antibody, Rabbit PAb ELISA 11213-RP01 100 µL  
11213-RP01 200 µL  
11213-RP01 400 µL  
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Anti-GST Tag Antibody Features

Features of Anti-GST Tag Antibodies:

High sensitivity:
• the dosage is only 1/20 compared with competitors
• detects lower amount of GST protein under same concentration compared with competitors

Suitable for different expression systems:
E.coli
• 293 cells
• insect cells

1. Transfected E.coli lysate: the dosage is only 1/20 compared with competitive brand

Sino Biological
0.04 μg/ml
Competitor A
0.8 μg/ml
WB -  Anti-GST Tag Mouse Monoclonal Antibody 0.04 μg/ml-Sino Biological WB -  Anti-GST Tag Mouse Monoclonal Antibody- 0.8 μg/ml Competitor A
Different loading quantity of His-GST-mBID transfected E.coli lysate
Sino Biological
0.04 μg/ml
Competitor A
0.8 μg/ml
WB - Anti-GST Tag Mouse Monoclonal Antibody 0.04 μg/ml-Sino Biological WB -  Anti-GST Tag Mouse Monoclonal Antibody- 0.8 μg/ml Competitor A
Different loading quantity of GST-3C-YWHAB transfected E.coli lysate

2. Transfected 293 cell lysate: detect lower amount of GST-tagged protein under same concentration compared with competitive brand

Sino Biological
0.8 μg/ml
Competitor A
0.8 μg/ml
WB -  Anti-GST Tag Mouse Monoclonal Antibody 0.8 μg/ml-Sino Biological WB -  Anti-GST Tag Mouse Monoclonal Antibody- 0.8 μg/ml Competitor A
Different loading quantity of FABP4-GST transfected 293 lysate
Sino Biological
0.8 μg/ml
Competitor A
0.8 μg/ml
WB - Anti-GST Tag Mouse Monoclonal Antibody 0.8 μg/ml-Sino Biological WB -  Anti-GST Tag Mouse Monoclonal Antibody- 0.8 μg/ml Competitor A
Different loading quantity of ARG1-GST transfected 293 lysate

3.Transfected insect cells lysate: the dosage is only 1/20 compared with competitive brand

Sino Biological
0.04 μg/ml
Competitor A
0.8 μg/ml
WB -  Anti-GST Tag Mouse Monoclonal Antibody 0.04 μg/ml-Sino Biological WB -  Anti-GST Tag Mouse Monoclonal Antibody- 0.8 μg/ml Competitor A
Different transfection time for GST tagged proteins transfected insect cell lysate

Compatible Secondary Antibodies:
> Goat Anti-Mouse IgG Secondary Antibody (HRP) (Cat: SSA007)
> Goat Anti-Mouse IgG-Fc Secondary Antibody (Biotin) (Cat: SSA013)
> Goat Anti-Rabbit IgG Secondary Antibody (Biotin) (Cat: SSA011)
> Goat Anti-Rabbit IgG-Fc Secondary Antibody (HRP) (Cat:SSA003)

Immunoprecipitation / IP Kit:
> GST Tag Immunomagnetic Beads (Cat: TB11213)

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