Anti-DYKDDDDK (FLAG® epitope Tag) Antibody

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Anti-DYKDDDDK (FLAG® epitope Tag) Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-DYKDDDDK (FLAG® epitope Tag) Antibody
Validated applications
WB,IP
Specificity
DYKDDDDK (FLAG® epitope Tag)
Immunogen
A synthetic peptide corresponding to the DYKDDDDK-tag sequence (CDYKDDDDK).
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, the synthetic peptide corresponding to the DYKDDDDK-tag sequence (CDYKDDDDK). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG2b Clone #05
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-DYKDDDDK (FLAG® epitope Tag) Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution
WB 1:500-1000
IP 0.1-0.5 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-DYKDDDDK (FLAG® epitope Tag) Antibody (Mouse Monoclonal antibody) Images

Flag tag was immunoprecipitated using:

Lane A:0.5 mg Flag-ARG1-GST transfected 293 Whole Cell Lysate

Lane B:0.5 mg GST-ARG1-flag transfected 293 Whole Cell Lysate

0.5 µL anti-Flag tag mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Flag tag mouse monoclonal antibody,at 1:1000 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 62 kDa

Observed band size: 62 kDa

Anti-Flag Tag mouse monoclonal antibody at 1:100 dilution

Lane A: IL12A-Fch+IL27B-Fc-Flag transfected 293 cell lysate(5ug)

Lane B: Non- transfected 293 Cells lysate(5ug)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Anti-Flag Tag mouse monoclonal antibody at 1:100 dilution

Lane A: Fc-flag Transfected 293 cell lysate(5ug)

Lane B: Non-transfected 293 Cells lysate(5ug)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Anti-Flag Tag mouse monoclonal antibody at 1:1000 dilution

Lane A: Flag-SMYD3-His Transfected 293 cell lysate(2.5ug)

Lane B: Non-transfected 293 Cells lysate(2.5ug)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

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