Anti-Bcl-2 Antibody

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Anti-Bcl-2 Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-Bcl-2 Antibody
Validated applications
WB,IHC-P
Species reactivity
Reacts with: Human
Specificity
Human Bcl-2
Immunogen
Recombinant Human Bcl-2 Protein (Catalog#10195-H08E)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Bcl-2 (rh Bcl-2; Catalog#10195-H08E; P10415; Met1-Asp211). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #17
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Bcl-2 Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
IHC-P 1:30-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Bcl-2 Antibody (Mouse Monoclonal antibody) Images

Anti-BCL2 mouse monoclonal antibody at 1:500 dilution

Lane A: Raji Whole Cell Lysate

Lane B: Jurkat Whole Cell Lysate

Lane C: K562 Whole Cell Lysate

Lane D: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Rabbit Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:26 kDa

Observed band size:26 kDa

Immunochemical staining of human BCL2 in human lymphoma with mouse monoclonal antibody at 1:60 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of human BCL2 in human lymph node with mouse monoclonal antibody at 1:60 dilution, formalin-fixed paraffin embedded sections.

Anti-Bcl-2 Antibody: Alternative Names

Anti-Bcl-2 Antibody; Anti-PPP1R50 Antibody

Bcl-2 Background Information

BCL2 (B-cell leukemia/lymphoma 2, N-Histidine-tagged), also known as Bcl-2, belongs to the Bcl-2 family. Bcl-2 family proteins regulate and contribute to programmed cell death or apoptosis. It is a large protein family and all members contain at least one of four BH (bcl-2 homology) domains. Certain members such as Bcl-2, Bcl-xl and Mcl1 are anti-apoptotic, whilst others are pro-apoptotic. Most Bcl-2 family members contain a C-terminal transmembrane domain that functions to target these proteins to the outer mitochondrial and other intracellular membranes. It is expressed in a variety of tissues. BCL2 blocks the apoptotic death of some cells such as lymphocytes. It also regulates cell death by controlling the mitochondrial membrane permeability and inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
Full Name
B-cell CLL/lymphoma 2
References
  • Tsujimoto Y, et al. (1984) Cloning of the chromosome breakpoint of neoplastic B cells with the t(14;18) chromosome translocation. Science. 226(4678):1097-99.
  • Cleary ML, et al. (1986) Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation. Cell. 47(1):19-28.
  • Otake Y, et al. (2007) Overexpression of nucleolin in chronic lymphocytic leukemia cells induces stabilization of Bcl-2 / Bcl-2 mRNA. Blood. 109(7):3069-75.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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