Anti-SOX2 Antibody

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Anti-SOX2 Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-SOX2 Antibody
Validated applications
WB,IHC-P,IP
Species reactivity
Reacts with: Human
Specificity
Human SOX2
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human Sox2.
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human Sox2, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-SOX2 Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
IHC-P 1:500-1:2000
IP 0.5-2 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-SOX2 Antibody (Rabbit Polyclonal antibody) Images

Immunochemical staining of human Sox2 in human glioma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (polypeptide).
Immunochemical staining of human Sox2 in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

Sox2 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-Sox2 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Sox2 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 37 kDa

Observed band size: 41 kDa

Anti-Sox2 rabbit polyclonal antibody at 1:500 dilution

Lane A: HESS9 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:34 kDa

Observed band size:37 kDa

(We are unsure as to the identity of these extra bands.)

Anti-SOX2 Antibody: Alternative Names

Anti-ANOP3 Antibody; Anti-MCOPS3 Antibody

SOX2 Background Information

SOX2, also known as ANOP3, is a transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP26. SOX2 is critical for early embryogenesis and for embryonic stem cell pluripotency. It keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Defects in SOX2 can cause microphthalmia, syndromic, 3. A disease characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
Full Name
SRY (sex determining region Y)-box 2
References
  • Lu Y, et al., 2010, PLoS ONE. 5(6): e11022.
  • Gontan C, et al., 2008, Dev. Biol. 317 (1): 296-309.
  • Shi W, et al., 2006, J. Biol. Chem. 281 (33): 23319-25.

Standard Antibody Development Service

Rabbit MAb

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Rabbit PAb

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Mouse MAb

Rabbit PAb

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