IL-6R ELISA Kit, Mouse

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IL-6R ELISA Kit, Mouse: General Information

Product name
IL-6R ELISA Kit, Mouse
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
1.48 pg/mL
Assay range
7.82-500 pg/mL
Specificity
Recognizes both recombinant and natural Mouse IL-6R
Interference
Preparations of the following factors at 50 ng/mL in a mid-range recombinant Mouse IL6Ra control were assayed for interference. No significant interference was observed.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 81.90 161.61 305.32
SD 5.93 9.85 15.95
CV(%) 7.2% 6.1% 5.2%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 79.84 150.47 286.47
SD 4.33 9.05 19.99
CV(%) 5.4% 6.0% 7.0%
Recovery
The recovery of Mouse IL6R spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
94
Range
86 -100%
Linearity
Serum
  Recovery of detected
1:2 93%
1:4 107%
1:8 101%
1:16 86%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This IL-6R ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-6R protein in Serum . It contains recombinant Mouse IL-6R, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

IL-6R ELISA Kit, Mouse: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Mouse IL6RA. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed. Cross-reactivity was observed to be 10% with 50 pg/mL of recombinant Rat IL6RA.

IL-6R ELISA Kit, Mouse: Alternative Names

CD126 ELISA Kit, Mouse; IL-6R ELISA Kit, Mouse; Il6r ELISA Kit, Mouse; MGC30256 ELISA Kit, Mouse

IL-6R Background Information

Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (sIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a sIL-6R protein are able to mediate IL-6 signals into the cells through the interaction of gp13. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp13 and inducing intracellular signalling. Through this so-called 'trans-signalling' mechanism, IL-6 is able to stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and sIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.
Full Name
interleukin 6 receptor
References
  • Barill S, et al. (2000) The role of interleukin-6 and interleukin-6/interleukin-6 receptor-alpha complex in the pathogenesis of multiple myeloma. Eur Cytokine Netw. 11(4): 546-51.
  • Kang KW, et al. (2007) Novel role of IL-6/SIL-6R signaling in the expression of inducible nitric oxide synthase (iNOS) in murine B16, metastatic melanoma clone F10.9, cells. Free Radic Biol Med. 42(2): 215-27.
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