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小鼠 C3a Receptor 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤

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Mouse C3AR1 cDNA Clone產品資訊
註冊碼:NM_009779.2
cDNA基因長度:1434bp
cDNA基因描述:Full length Clone DNA of Mus musculus complement component 3a receptor 1 with C terminal OFPSpark / RFP tag.
基因別名:AZ3B, C3AR, HNFAG09, C3ar1
分子種屬:Mouse
載體:pCMV3-C-OFPSpark
Plasmid:
限制性酶切位點:
Tag序列:OFPSpark Tag: GATAGCACTGAG……CACCTGTTCCAG
序列資訊:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
OFP (OFPSpark) / RFP Tag Info

OFPSpark is a red (orange) fluorescent protein (excitation/emission maxima are 549 and 566 nm, respectively) derived from DsRed. Possessing high photostability and pH stability, OFPSpark is more than twice brighter than mOrange2. Fast OFPSpark maturation makes it clearly detectable in mammalian cells as early as within 8 hrs after transfection. OFPSpark can be expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with OFPSpark expression vectors produce bright fluorescence in 8 hrs after transfection. No cytotoxic effects or visible protein aggregation are observed. For its monomer structure, OFPSpark performs well in some fusions and protein labeling applications.

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貨號: MG50419-ACR
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