MFGE8 cDNA ORF Clone in Cloning Vector, Human

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MFGE8 cDNA ORF Clone in Cloning Vector, Human: General Information

Gene
Species
Human
NCBI Ref Seq
RefSeq ORF Size
1164 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence except for the point mutation 1155 G/A not causing the amino acid variation.
Description
Full length Clone DNA of Human milk fat globule-EGF factor 8 protein.
Plasmid
Sequencing Primers
M13-47 and RV-M
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Ampicillin
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.

MFGE8 cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

MFGE8 cDNA ORF Clone in Cloning Vector, Human: Alternative Names

BA46 cDNA ORF Clone, Human; EDIL1 cDNA ORF Clone, Human; HMFG cDNA ORF Clone, Human; hP47 cDNA ORF Clone, Human; HsT19888 cDNA ORF Clone, Human; MFG-E8 cDNA ORF Clone, Human; MFGM cDNA ORF Clone, Human; OAcGD3S cDNA ORF Clone, Human; SED1 cDNA ORF Clone, Human; SPAG10 cDNA ORF Clone, Human

MFGE8 Background Information

MFG-E8, also known as lactadherin and MFGE8, contains 1 EGF-like domain and 2 F5/8 type C domains. It also contains a phosphatidylserine (PS) binding domain, as well as an Arginine-Glycine-Aspartic acid motif, which enables the binding to integrins. It binds PS, which is exposed on the surface of apoptotic cells. MFG-E8 is expressed in mammary epithelial cell surfaces and aortic media. Overexpression of MFG-E8 can be found in several carcinomas. MFG-E8 has an opsonization of the apoptotic cells and binding to integrins on the surface of phagocytic cells. It also mediates the engulfment of the dead cell. MFG-E8 plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. It promotes VEGF-dependent neovascularization and contributes to phagocytic removal of apoptotic cells in many tissues. It also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner.
Full Name
milk fat globule-EGF factor 8 protein
References
  • Oshima K, et al. (2002) Secretion of a peripheral membrane protein, MFG-E8, as a complex with membrane vesicles. Eur J Biochem. 269(4):1209-18.
  • Hanayama R, et al. (2002) Identification of a factor that links apoptotic cells to phagocytes. Nature. 417 (6885):182-7.
  • Strausberg RL, et al. (2003) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci. 99(26):16899-903.
  • Phosphatidylserine exposure mediated by ABC transporter activates the integrin signaling pathway promoting axon regeneration
    Author
    Hisamoto, N;Tsuge, A;Pastuhov, SI;Shimizu, T;Hanafusa, H;Matsumoto, K;
    Year
    2018
    Journal
    Nat Commun
    Application
    template
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