L1CAM Protein, Human, Recombinant (His Tag)

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L1CAM Protein, Human, Recombinant (His Tag): Product Information

Purity
> 85 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the extracellular domain of human CD171 (NP_000416.1) (Met 1-Glu 1120) was fused with a polyhistidine tag at the C-terminus.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Ile 20
Molecule Mass
The recombinant human CD171 consists of 1112 amino acids after removal of the signal peptide and predicts a molecular mass of 125 kDa. As a result of glycosylation, the apparent molecular mass of rhCD171 is approximately 160-200 kDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

L1CAM Protein, Human, Recombinant (His Tag): Images

L1CAM Protein, Human, Recombinant (His Tag): Alternative Names

CAML1 Protein, Human; CD171 Protein, Human; HSAS Protein, Human; HSAS1 Protein, Human; MASA Protein, Human; MIC5 Protein, Human; N-CAM-L1 Protein, Human; N-CAML1 Protein, Human; NCAM-L1 Protein, Human; S10 Protein, Human; SPG1 Protein, Human

L1CAM Background Information

L1 cell adhesion molecule (L1CAM), also designated as CD171, is a cell adhesion receptor of the immunoglobulin superfamily, known for its roles in nerve cell function. While originally believed to be present only in brain cells, in recent years L1-CAM has been detected in other tissues, and in a variety of cancer cells, including some common types of human cancer. L1CAM interacts with a variety of ligands including axonin-1, CD9, neurocan and intergrins, and it has been revealed that the RGD motif in the sixth Ig domain of L1CAM is a binding site for integrins, thus important for nuclear signaling. Disruption of L1CAM function causes three X-linked neurological syndromes, i.e. hydrocephalus, MASA syndrome (mental retardation, aphasia, shuffling gait and adducted thumbs) and spastic paraplegia syndrome. Overexpression of L1CAM in normal and cancer cells increased motility, enhanced growth rate and promoted cell transformation and tumorigenicity. Recent work has identified L1CAM (CD171) as a novel marker for human carcinoma progression, and a candidate for anti-cancer therapy.
Full Name
L1 cell adhesion molecule
References
  • Meier F, et al. (2006) The adhesion molecule L1 (CD171) promotes melanoma progression. Int J Cancer. 119(3): 549-55.
  • Gavert N, et al. (2008) L1-CAM in cancerous tissues. Expert Opin Biol Ther. 8(11): 1749-57.
  • Issa Y, et al. (2009) Enhanced L1CAM expression on pancreatic tumor endothelium mediates selective tumor cell transmigration. J Mol Med. 87(1): 99-112.
  • Weidle UH, et al. (2009) L1-CAM as a target for treatment of cancer with monoclonal antibodies. Anticancer Res. 29(12): 4919-31.
  • Raveh S, et al. (2009) L1 cell adhesion molecule (L1CAM) in invasive tumors. Cancer Lett. 282(2): 137-45.
  • Wolterink S, et al. (2010) Therapeutic antibodies to human L1CAM: functional characterization and application in a mouse model for ovarian carcinoma. Cancer Res. 70(6): 2504-15.
  • Angiogenic Cytokines Are Antibody Targets During Graft-versus-Leukemia Reactions
    Author
    Piesche, M;Ho, VT;Kim, H;Nakazaki, Y;Nehil, M;Yaghi, NK;Kolodin, D;Weiser, J;Altevogt, P;Kiefel, H;Alyea, EP;Antin, JH;Cutler, C;Koreth, J;Canning, C;Ritz, J;Soiffer, RJ;Dranoff, G;
    Year
    2015
    Journal
    Clin. Cancer Res.
    Application
    ELISA (coating protein)
  • Circulating levels of autoantibodies against L1-cell adhesion molecule as a potential diagnostic biomarker in esophageal squamous cell carcinoma
    Author
    Xu, YW;Peng, YH;Ran, LQ;Zhai, TT;Guo, HP;Qiu, SQ;Chen, HL;Wu, ZY;Li, EM;Xie, JJ;
    Year
    2017
    Journal
    Clin Transl Oncol
    Application
    ELISA
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