Influenza H5N1 Hemagglutinin / HA Neutralizing Antibody

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Influenza H5N1 Hemagglutinin / HA Neutralizing Antibody ( antibody) General Information

Product name
Influenza H5N1 Hemagglutinin / HA Neutralizing Antibody
Validated applications
Microneutralizaiton(MN),HemagglutininInhibition(HI) (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
Influenza Hemagglutinin / HA
Source
Human IgG1 Clone #H011
Formulation
0.2 μm filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH6.0
Conjugate
Unconjugated
Endotoxin
< 3 EU/mg
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Influenza H5N1 Hemagglutinin / HA Neutralizing Antibody ( antibody) Validated Applications

Notes
Hemagglutinin Inhibition (HI): This H5N1 HA protein neutralizing antibody can inhibit the hemagglutinin activity of recombinant H5N1 HA protein, the HI titer is 0.05-0.2 µg/mL When 1 unit of protein added.
Microneutralizaiton (MN): The influenza A H5N1 HA protein Neutralizing antibody can effectively neutralize the H5N1 pseduotype virus which contain HIV backbone and H5N1 HA protein from infecting 293FT cell. The pseudovirus microneutralizaiton (MN) test was performed on 293FT cells infected with H5N1 (A/Vietnam/1194/2004) pseudovirus under the treatment of serial dilution of neutralizing antibody. Percentage of inhibition is 100% with 1 µg/mL influenza A H5N1 HA neutralizing Antibody incubated with high titer of H5N1 pse
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Hemagglutinin / HA Background Information

The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Full Name
Harvey rat sarcoma viral oncogene homolog
References
  • White JM, Hoffman LR, Arevalo JH, et al. (1997). ""Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin"". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.
  • Suzuki Y (March 2005). ""Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses"". Biol. Pharm. Bull. 28 (3): 399–408.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). ""Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential"". Avian Dis. 40 (2): 425–37
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