Full length Clone DNA of Influenza A H5N1 (A/Thailand/1(KAN-1)/2004) Hemagglutinin with C terminal His tag.
基因別名:
Hemagglutinin, HA
分子種屬:
H5N1
載體:
pCMV3-C-His
Plasmid:
pCMV3-H5N1-TH-04-HA-His
限制性酶切位點:
KpnI + XbaI (6kb + 1.75kb)
Tag序列:
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
序列資訊:
A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with AFF60787.1.
Sequencing primers:
T7(TAATACGACTCACTATAGGG)
BGH(TAGAAGGCACAGTCGAGG)
Promoter:
Enhanced CMV mammalian cell promoter
Application:
Stable or Transient mammalian expression
Antibiotic in E.coli:
Kanamycin
Antibiotic in mammalian cell:
Hygromycin
運輸條件:
Each tube contains lyophilized plasmid.
儲存方法:
The lyophilized plasmid can be stored at ambient temperature for three months.
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.
甲型流感 H5N1 (Thailand/1(KAN-1)/2004) 血凝素 (Hemagglutinin / HA) (Codon Optimized) ORF mammalian expression plasmid, C-His 標籤 on other vectors
The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA0, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA0) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
參考資料
White JM, Hoffman LR, Arevalo JH, et al. (1997). "Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.
Suzuki Y (March 2005). "Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses". Biol. Pharm. Bull. 28 (3): 399–408.
Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). "Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential". Avian Dis. 40 (2): 425–37
