IL1R1 was immunoprecipitated using:
Lane A:0.5 mg Raw264.7 Whole Cell Lysate2 µL anti-IL1R1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-IL1R1 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:
Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilutionDeveloped using the DAB staining technique.
Performed under reducing conditions.Predicted band size: 65 kDa
Observed band size: 65 kDa
Anti-IL1R1 rabbit polyclonal antibody at 1:500 dilution
Lane A: RAW264.7Membrane LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:65 kDa
Observed band size:65 kDa
(We are unsure as to the identity of these extra bands.)