Anti-IL-18 Antibody

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Anti-IL-18 Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-IL-18 Antibody
Validated applications
ELISA,ELISA(Cap)
Species reactivity
Reacts with: Human
Specificity
Human IL-18
Immunogen
Recombinant Human IL-18 protein (Catalog#10119-H09E)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human IL-18 (rh IL-18; Catalog#10119-H09E; Q14116; Met 1-Asp193).
Source
Monoclonal Rabbit IgG Clone #011
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-IL-18 Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution
ELISA 1:5000-1:10000
ELISA(Cap) 1:250-1:2000
Notes
ELISA(Cap): This antibody will detect Human IL-18 in ELISA pair set (Catalog: # SEK10119). In a sandwich ELISA, it can be used as capture antibody.
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-IL-18 Antibody: Alternative Names

Anti-IGIF Antibody; Anti-IL-18 Antibody; Anti-IL-1g Antibody; Anti-IL1F4 Antibody; Anti-Interleukin 18 Antibody

IL-18 Background Information

Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1−like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cells-, IL-4− and STAT6−dependent fashion. IL-18 and T cell receptor−mediated stimulation could induce naïve CD4+ T cells to develop into IL-4−producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.
Full Name
interleukin 18
References
  • Dinarello CA. (1999) IL-18: A TH1-inducing, proinflammatory cytokine and new member of the IL-1 family. J Allergy Clin Immunol. 103: 11-24.
  • Takeda K, et al.. (1998) Defective NK cell activity and Th1 response in IL-18-deficient mice. Immunity. 8(3): 383-90.
  • Gracie JA, et al.. (1999) A proinflammatory role for IL-18 in rheumatoid arthritis. J Clin Invest. 104(10): 1393-401.
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