Human respiratory syncytial virus (RSV) Fusion Glycoprotein / RSV-F ELISA Pair Set General Information
Human respiratory syncytial virus (RSV) Fusion Glycoprotein / RSV-F ELISA Pair Set
Solid Phase Sandwich ELISA
Quantitative determination of RSV RSV Fusion
1. Capture Antibody 1.0 mg/mL of rabbit anti-RSV-F monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 11049-R338) 2. Detection Antibody 0.2 mg/mL rabbit anti-RSV-F monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. (Catalog: # 11049-R302) 3. Standard Each vial contains 230 ng of recombinant RSV-F. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 8 ng/mL is recommended.
This Human respiratory syncytial virus (RSV) Fusion Glycoprotein / RSV-F ELISA Pair Set is a solid phase sandwich ELISA for quantitative determination of RSV RSV Fusion . It contains RSV RSV Fusion capture antibody, RSV RSV Fusion detector antibody
and a highly purified
recombinant RSV RSV Fusion protein. This Pair Set is at affordable price for researchers.
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE! Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Human respiratory syncytial virus (RSV) Fusion Glycoprotein / RSV-F ELISA Pair Set Images
Human respiratory syncytial virus (RSV) Fusion Glycoprotein / RSV-F ELISA Pair Set: Alternative Names
Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. It is classified within the genus pneumovirus of the family paramyxoviridae. Like other members of the family, HRSV has two major surface glycoproteins (G and F) that play important roles in the initial stages of the infectious cycle. The G protein mediates attachment of the virus to cell surface receptors, while the F protein promotes fusion of the viral and cellular membranes, allowing entry of the virus ribonucleoprotein into the cell cytoplasm. The fusion (F) protein of RSV is synthesized as a nonfusogenic precursor protein (F), which during its migration to the cell surface is activated by cleavage into the disulfide-linked F1 and F2 subunits. This fusion is pH independent and occurs directly at the outer cell membrane, and the F2 subunit was identifed as the major determinant of RSV host cell specificity. The trimer of F1-F2 interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and induces the fusion between host cell and virion membranes. Notably, RSV fusion protein is unique in that it is able to interact directly with heparan sulfate and therefore is sufficient for virus infection. Furthermore, the fusion protein is also able to trigger p53-dependent apoptosis.
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