MMP-9 Matched ELISA Antibody Pair Set,Human

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MMP-9 Matched ELISA Antibody Pair Set,Human General Information

Product name
MMP-9 Matched ELISA Antibody Pair Set,Human
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Human MMP-9
Assay range
31.25-2000 pg/mL
Materials provided
1. Capture Antibody 0.5 mg/mL of mouse anti-Human MMP9/MMP-9/CLG4B monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10327-MM06)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human MMP9/MMP-9/CLG4B monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10327-R043)
3. Standard Each vial contains 145 ng of recombinant Human MMP9/MMP-9/CLG4B. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2000 pg/mL is recommended.
Product overview
This MMP-9 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human MMP-9 . It contains Human MMP-9 capture antibody, Human MMP-9 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human MMP-9 protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

MMP-9 Matched ELISA Antibody Pair Set,Human Images

MMP-9 Matched ELISA Antibody Pair Set,Human: Alternative Names

CLG4B Matched ELISA Antibody Pair Set, Human; GELB Matched ELISA Antibody Pair Set, Human; MANDP2 Matched ELISA Antibody Pair Set, Human; MMP-9 Matched ELISA Antibody Pair Set, Human

MMP-9 Background Information

Matrix metalloproteinases (MMPs) are neutral proteinases that are involved in the breakdown and remodeling of the extracellular matrix (ECM) under a variety of physiological and pathological conditions, such as morphogenesis, differentiation, angiogenesis and tissue remodeling, as well as pathological processes including inflammation, arthritis, cardiovascular diseases, pulmonary diseases and tumor invasion. MMP9, also known as 92-kDa gelatinase B/type IV collagenase, is secreted from neutrophils, macrophages, and a number of transformed cells, and is the most complex family member in terms of domain structure and regulation of its activity. It plays an important role in tissue remodelling in normal and pathological inflammatory processes. MMP-9 is a major secretion product of macrophages and a component of cytoplasmic granules of neutrophils, and is particularly important in the pathogenesis of inflammatory, infectious, and neoplastic diseases in many organs including the lung. This enzyme is also secreted by lymphocytes and stromal cells upon stimulation by inflammatory cytokines, or upon delivery of bi-directional activation signals following integrin-mediated cell-cell or cell-extracellular matrix (ECM) contacts. Since the integrity of the tissue architecture is closely dependent of the delicate balance between MMPs and their inhibitors, excessive production of MMP-9 is linked to tissue damage and degenerative inflammatory disorders. As a consequence, regulation of gene transcription and tissue-specific expression of MMP-9 in normal and diseased states are being actively investigated to pave the way for new therapeutic targets. In addition, the dramatic overexpression of MMP-9 in cancer and various inflammatory conditions clearly points to the molecular mechanisms controlling its expression as a potential target for eventual rational therapeutic intervention.
Full Name
matrix metallopeptidase 9
References
  • St-Pierre Y, et al. (2003) Emerging features in the regulation of MMP-9 gene expression for the development of novel molecular targets and therapeutic strategies. Curr Drug Targets Inflamm Allergy. 2(3): 206-15.
  • St-Pierre Y, et al. (2004) Regulation of MMP-9 gene expression for the development of novel molecular targets against cancer and inflammatory diseases. Expert Opin Ther Targets. 8(5): 473-89.
  • Chakrabarti S, et al. (2005) Matrix metalloproteinase-2 (MMP-2) and MMP-9 in pulmonary pathology. Exp Lung Res. 31(6): 599-621.
  • Matrix metalloproteinase 9 gene polymorphism 1562C>T is significantly associated with acute coronary syndrome susceptibility in the Vietnamese population
    Author
    Bich Thao Le, T;Huyen Bui, T;Minh Phuong Nguyen, T;Minh Nguyet Tran, T;Chi Do, H;Dung Nguyen, T;Dinh Pham, M;Loi Do, D;Nhi Nguyen, B;Chi Phan, V;
    Year
    2018
    Journal
    ScienceAsia
    Application
    ELISA
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