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人 KIT / c-KIT / CD117 ELISA Kit

  • Human KIT / c-KIT / CD117 ELISA Kit, TYPICAL DATA
  • Human KIT / c-KIT / CD117 ELISA Kit, SPECIFICITY
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檢測原理
Solid Phase Sandwich ELISA
預期用途
For the quantitative determination of Human KIT / c-KIT / CD117 concentration in serum.
The use of this kit for other sample types need be validated by the end user due to the complexity of natural targets and unpredictable interference.
線性範圍
39-2500 pg/mL
靈敏度
The minimum detectable dose of Human KIT / c-KIT / CD117 is typically less than 5.8 pg/mL. The MDD was determined by adding three standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
試劑盒組分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
儲存方法
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
 KIT ELISA Kit Standard Curve
Human KIT / c-KIT / CD117 ELISA Kit, TYPICAL DATA
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Caption:
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
 KIT ELISA Kit Specificity
Human KIT / c-KIT / CD117 ELISA Kit, SPECIFICITY
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Caption:
This assay recognizes both recombinant and natural Human KIT. The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
Preparations of FLT3、MCSFR、PDGFRα、PDGFRβ、VEGFR1、VEGFR2 and VEGFR3 at 25 ng/mL in a mid-range recombinant human KIT control were assayed for interference. No significant interference was observed.Recombinant human SCF does not cross-react in this assay but does interfere at concentrations greater than 5 ng/mL.

KIT / c-KIT / CD117 研究背景

C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains.and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family and CSF-1/PDGF receptor subfamily. C-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. C-Kit has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumour in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumours and in distinguishing seminomas from embryonal carcinomas. Mutations in c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

 KIT / c-KIT / CD117 參考資料
  • Andre C, et al. (1997) Sequence analysis of two genomic regions containing the KIT and the FMS receptor tyrosine kinase genes. Genomics. 39(2):216-26.
  • Yarden Y, et al. (1987) Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand. EMBO J. 6(11):3341-51.
  • Leong KG, et al. (2008) Generation of a prostate from a single adult stem cell. Nature. 456(7223): 804-8.
  • Edling CE, et al. (2007) c-Kit--a hematopoietic cell essential receptor tyrosine kinase. Int J Biochem Cell Biol. 39(11):1995-8.
  • McIntyre A, et al. (2005) Amplification and overexpression of the KIT gene is associated with progression in the seminoma subtype of testicular germ cell tumors of adolescents and adults. Cancer Res. 65(18):8085-9.
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    貨號: KIT11996-1
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