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人 EEBB3/HER3 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤

  • Human ErbB3 / HER3 transcript variant 1 Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-tagged
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 ERBB3 cDNA Clone產品資訊
註冊碼:NM_001982.2
cDNA基因長度:4029bp
cDNA基因描述:Full length Clone DNA of Homo sapiens v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) , transcript variant 1 with C terminal Myc tag.
基因別名:HER3, LCCS2, ErbB-3, c-erbB3, erbB3-S, MDA-BF-1, MGC88033, c-erbB-3, p180-ErbB3, p45-sErbB3, p85-sErbB3, ERBB3
分子種屬:Human
載體:pCMV3-C-Myc
Plasmid:pCMV3-ERBB3-Myc
限制性酶切位點:KpnI + NotI (6kb + 4.08kb)
Tag序列:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
序列資訊:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with ERBB3 qPCR primers for gene expression analysis, HP100075 )
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
 ERBB3 Gene Plasmid Map
Human ErbB3 / HER3 transcript variant 1 Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-tagged
Flow cytometric analysis of Human CD32 expression on human whole blood lymphocytes (left panel), monocytes (middle panel) and granulocytes (right panel). Cells were stained with purified anti-Human CD32, then a FITC-conjugated second step antibody. The histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes, monocytes and granulocytes.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

人 EEBB3/HER3 transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤 on other vectors
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研究背景

ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

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