EGFR Protein, Human, Recombinant (aa 668-1210, His & GST Tag)

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EGFR Protein, Human, Recombinant (aa 668-1210, His & GST Tag): Product Information

Purity
> 85 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Activity
The specific activity was determined to be 105 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate (see Activity Assay Protocol)
Protein Construction
A DNA sequence encoding the cytoplasmic domain (Met 668-Ala 1210) of human EGFR (NP_005219) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Accession#
Expressed Host
Baculovirus-Insect Cells
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human EGFR /GST chimera consists of 780 amino acids and has a calculated molecular mass of 89.1 kDa.
Formulation
Supplied as sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

EGFR Protein, Human, Recombinant (aa 668-1210, His & GST Tag): Images

EGFR Protein, Human, Recombinant (aa 668-1210, His & GST Tag): Alternative Names

ERBB Protein, Human; ERBB1 Protein, Human; HER1 Protein, Human; mENA Protein, Human; NISBD2 Protein, Human; PIG61 Protein, Human

EGFR Background Information

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
Full Name
epidermal growth factor receptor
References
  • Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
  • Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
  • Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.
  • EGFR induces DNA decomposition via phosphodiester bond cleavage
    Author
    Tong, Y;Li, S;Huang, C;
    Year
    2017
    Journal
    Sci Rep
    Application
    enzyme (DNA degradation)
  • Label-free real-time ultrasensitive monitoring of non-small cell lung cancer cell interaction with drugs
    Author
    Dai, H;Jiao, Y;Sun, Z;Cao, Z;Chen, X;
    Year
    2018
    Journal
    Biomed. Opt. Express
    Application
    binding
  • Automated literature mining and hypothesis generation through a network of Medical Subject Headings
    Author
    Wilson, S;Wilkins, A;Holt, M;Choi, B;Konecki, D;Lin, C;Koire, A;Chen, Y;Kim, S;Wang, Y;Wastuwidyaningtyas, B;Qin, J;Donehower, L;Lichtarge, O;
    Year
    2018
    Journal
    bioRxiv
    Application
    enzyme (kinase)
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