Anti-EGFR Antibody

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Anti-EGFR Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-EGFR Antibody
Validated applications
ELISA,IHC-P,IHC-Fr,ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human EGFR
No cross-reactivity in ELISA with
Human ErbB2
Human ErbB3
Immunogen
Recombinant Human EGFR / HER1 / ErbB1 protein (Catalog#10001-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human EGFR / HER1 / ErbB1 (rh EGFR / HER1 / ErbB1; Catalog#10001-H08H; NP_005219; Met 1-Ser 645).
Source
Monoclonal Rabbit IgG Clone #043
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-EGFR Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution
ELISA 1:5000-1:10000
IHC-P 1:50-1:200
IHC-Fr 1:50-1:200
ICC-IF 1:20-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-EGFR Antibody (Rabbit Monoclonal antibody) Images

Immunofluorescence staining of EGFR in monkey skin with rabbit monoclonal antibody (1:50, frozen section). The image showing membrane staining of squamous epithelium cell.
Immunochemical staining of human EGFR in human uterine cervix cancer with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to uterine gland.
Immunochemical staining of human EGFR in human rectal cancer with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to rectal gland. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
Immunochemical staining of human EGFR in human placenta with rabbit monoclonal antibody (1:200,formalin-fixed paraffin embedded sections). Positive staining was localized to trophoblast.
Immunofluorescence staining of EGFR in A431 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human EGFR monoclonal antibody (dilution ratio 1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Cell membrane.

Anti-EGFR Antibody: Alternative Names

Anti-ERBB Antibody; Anti-ERBB1 Antibody; Anti-HER1 Antibody; Anti-mENA Antibody; Anti-NISBD2 Antibody; Anti-PIG61 Antibody

EGFR Background Information

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
Full Name
epidermal growth factor receptor
References
  • Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
  • Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
  • Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.
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