C5a Matched ELISA Antibody Pair Set,Human

1/1

This product is not available, please contact order_us@sinobiological.com for relevant product if required.

C5a Matched ELISA Antibody Pair Set,Human General Information

Product name
C5a Matched ELISA Antibody Pair Set,Human
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Human C5a
Assay range
31.25-2000 pg/mL
Materials provided
1. Capture Antibody 1 mg/mL of mouse anti-Human Complement C5a monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10604-MM06)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human Complement C5a polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 100 ng of recombinant Human Complement C5a . Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2000 pg/mL is recommended.
Product overview
This C5a Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human C5a . It contains Human C5a capture antibody, Human C5a detector antibody (HRP) and a highly purified E. coli-expressed recombinant Human C5a protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

C5a Matched ELISA Antibody Pair Set,Human Images

C5a Matched ELISA Antibody Pair Set,Human: Alternative Names

C5a Matched ELISA Antibody Pair Set, Human; C5b Matched ELISA Antibody Pair Set, Human; C5D Matched ELISA Antibody Pair Set, Human; C5Da Matched ELISA Antibody Pair Set, Human; Complement 5a Matched ELISA Antibody Pair Set, Human; CPAMD4 Matched ELISA Antibody Pair Set, Human; ECLZB Matched ELISA Antibody Pair Set, Human

C5a Background Information

C5a is a protein fragment released from complement component C5. This 74 amino acid peptide in humans is generated by the cleavage of C5a convertase on the C5 α-chain during the classical, alternative, and lectin pathways of complement activation. The structure of C5a includes a core region consisting of four, anti-parallel alpha-helices held together by three disulfide linkages and a structured C-terminal tail, and C5a is rapidly metabolised by carboxypeptidase B to a 73 amino acid low activity form, C5a des-Arg. C5a is an extremely potent proinflammatory mediator, as well as a potent chemotactic factor for neutrophils and other leukocytes. It causes histamine release, increases in vascular permeability, induces several cytokines production from leukocytes, enhances neutrophil-endothelial cell adhesion, and augments the humoral and cell-mediated immune response. C5a is quickly metabolised by carboxypeptidases, forming the less potent C5adesArg. Acting via a classical G protein-coupled receptor, CD88, C5a and C5adesArg exert a number of effects essential to the innate immune response, while their actions at the more recently discovered non-G protein-coupled receptor, C5L2 (or GPR77), remain unclear. The widespread expression of C5a receptors throughout the body allows C5a to elicit a broad range of effects. Thus, C5a has been found to be a significant pathogenic driver in a number of immuno-inflammatory diseases, making C5a inhibition an attractive therapeutic strategy. C5a is a strong chemoattractant and is involved in the recruitment of inflammatory cells such as neutrophils, eosinophils, monocytes, and T lymphocytes, in activation of phagocytic cells and release of granule-based enzymes and generation of oxidants, all of which may contribute to innate immune functions or tissue damage. Accordingly, the anaphylatoxin C5a is implicated in a variety of diseases such as rheumatoid arthritis, systemic lupus erythematosus, reperfusion injury, Alzheimer's disease, and sepsis.
Full Name
complement component 5
References
  • Guo RF, et al.. (2005) Role of C5a in inflammatory responses. Annu Rev Immunol. 23: 821-52.
  • Guo RF, et al. (2006) C5a, a therapeutic target in sepsis. Recent Pat Antiinfect Drug Discov. 1(1): 57-65.
  • Manthey HD, et al. (2009) Complement component 5a (C5a). Int J Biochem Cell Biol. 41(11): 2114-7.
  • Differential activation mechanisms of serum C5a in lupus nephritis and neuropsychiatric systemic lupus erythematosus
    Author
    Sakuma, Y;Nagai, T;Yoshio, T;Hirohata, S;
    Year
    2016
    Journal
    Mod Rheumatol
    Application
    ELISA
Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait