CEACAM5 Protein, Human, Recombinant (His & Fc Tag)

1/1
Price:
Size:
Number:

CEACAM5 Protein, Human, Recombinant (His & Fc Tag): Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the mature form of human CEACAM5 (NP_004354.2) (Met 1-Ala 685) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Lys 35
Molecule Mass
The recombinant human CEACAM5/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 899 amino acids and has a predicted molecular mass of 99.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCEACAM5/Fc monomer is approximately 160-180 kDa due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CEACAM5 Protein, Human, Recombinant (His & Fc Tag): Images

CEACAM5 Protein, Human, Recombinant (His & Fc Tag): Alternative Names

CD66e Protein, Human; CEA Protein, Human

CEACAM5 Background Information

CEACAM5, also known as CEA or D66e, belongs to the large CEACAM subfamily of immunoglobulin superfamily. CEACAM5 is expressed primarily by epithelial cells, and is synthesized as a glycoprotein with a MW of 18 kDa comprising 6% carbohydrate. CEACAM5 contains one Ig-like V-type domain at the N-terminus, followed by six Ig-like C2-type domain and a GPI anchor, and exists as a homodimer. CEACAM5 and CEACAM6 are overexpressed in many cancers and are associated with adhesion and invasion. CEACAM5 can mediate cell-cell adhesion through homotypic and heterotypic interactions. It functions as a homotypic intercellular adhesion molecule and serves as a widely used tumor marker, since it is expressed at higher levels in tumorous tissues than in corresponding normal tissues. CEACAM5 has also been shown to contribute to tumorigenicity by inhibiting cellular differentiation. In addition, CEACAM5 is identified as the host receptor for the Dr family of adhesins of E.Coli, and the binding of E.coli Dr adhesins leads to dissociation of the CEACAM5 homodimer.
Full Name
carcinoembryonic antigen-related cell adhesion molecule 5
References
  • Baczyska D, et al. (2003) The tumorigenic potential of human CX-1 colon adenocarcinoma cells depends on carcinoembryonic antigen (CEACAM5) expression. Cell Mol Biol Lett. 8(2): 471-86.
  • Blumenthal RD, et al. (2005) Inhibition of adhesion, invasion, and metastasis by antibodies targeting CEACAM6 (NCA-90) and CEACAM5 (Carcinoembryonic Antigen). Cancer Res. 65(19): 8809-17.
  • Liebig B, et al. (2005) Forced expression of deltaN-TCF-1B in colon cancer derived cell lines is accompanied by the induction of CEACAM5/6 and mesothelin. Cancer Lett. 223(1): 159-67.
  • Helicobacter pylori adhesin HopQ engages in a virulence-enhancing interaction with human CEACAMs
    Author
    Javaheri, A;Kruse, T;Moonens, K;Luque, RM;Debraekeleer, A;Asche, CI;Tegtmeyer, N;Kalali, B;Bach, NC;Sieber, SA;Hill, DJ;Königer, V;Hauck, CR;Moskalenko, R;Haas, R;Busch, DH;Klaile, E;Slevogt, H;Schmidt, A;Backert, S;Remaut, H;Singer, BB;Gerhard, M;
    Year
    2016
    Journal
    Nature microbiology
    Application
    binding
  • Binding of Candida albicans to Human CEACAM1 and CEACAM6 Modulates the Inflammatory Response of Intestinal Epithelial Cells
    Author
    Klaile, E;Müller, MM;Schäfer, MR;Clauder, AK;Feer, S;Heyl, KA;Stock, M;Klassert, TE;Zipfel, PF;Singer, BB;Slevogt, H;
    Year
    2017
    Journal
    MBio
    Application
    binding
Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait