Anti-Thy1 / CD90 Antibody

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Anti-Thy1 / CD90 Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-Thy1 / CD90 Antibody
Validated applications
WB,IHC-P,IP
Species reactivity
Reacts with: Mouse
Specificity
Mouse Thy1 / CD90
Immunogen
Recombinant Mouse CD90/THY-1 Protein (Catalog#50461-M08H)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse CD90/THY-1 (rM CD90/THY-1; Catalog#50461-M08H; NP_033408.1; Met1-Cys131). CD90/THY-1 specific IgG was purified by Mouse CD90/THY-1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Thy1 / CD90 Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
IHC-P 1:200-1:2000
IP IP:0.5-2 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Thy1 / CD90 Antibody (Rabbit Polyclonal antibody) Images

Anti-THY1 rabbit polyclonal antibody at 1:500 dilution

Lane A: Brain(mouse) Whole Cell Lysate

Lane B: kidney(mouse) Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:18 kDa

Observed band size:23 kDa

Immunochemical staining of mouse THY1 in mouse brain with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of mouse THY1 in mouse spleen with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.

mTHY1 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-mTHY1 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-mTHY1 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution

Developed using the technique.

Performed under reducing conditions.

Predicted band size: 18 kDa

Observed band size :25 kDa

Anti-Thy1 / CD90 Antibody: Alternative Names

Anti-CD90 Antibody; Anti-T25 Antibody; Anti-Thy-1 Antibody; Anti-Thy-1.2 Antibody; Anti-Thy1.1 Antibody; Anti-Thy1.2 Antibody

Thy1 / CD90 Background Information

Thy-1 membrane glycoprotein, also known as Thy-1 antigen, CD9 and THY1, is a cell membrane protein which contains 1 Ig-like V-type (immunoglobulin-like) domain. It is a glycophosphatidylinositol-linked glycoprotein expressed on the surface of neurons, thymocytes, subsets of fibroblasts, endothelial cells, mesangial cells and some hematopoietic cells. It has been identified on a variety of stem cells and at varying levels in non-lymphoid tissues such as on fibroblasts, brain cells, and activated endothelial cells. Thy-1 is evolutionarily conserved, developmentally regulated, and often has dramatic effects on cell phenotype. Thy-1 is a 25-37 kDa glycosylphosphatidylinositol (GPI)-anchored protein involved in T cell activation, neurite outgrowth, apoptosis, tumor suppression, wound healing, and fibrosis. To mediate these diverse effects, Thy-1 participates in multiple signaling cascades. Thy-1 is an important regulator of cell-cell and cell-matrix interactions, with important roles in nerve regeneration, metastasis, inflammation, and fibrosis.
Full Name
Thy-1 cell surface antigen
References
  • Rege TA, et al. (2006) Thy-1 as a regulator of cell-cell and cell-matrix interactions in axon regeneration, apoptosis, adhesion, migration, cancer, and fibrosis. FASEB J. 20(8): 1045-54.
  • Fiegel HC, et al. (2008) Lack of Thy1 (CD90) expression in neuroblastomas is correlated with impaired survival. Pediatr Surg Int. 24(1): 101-5.
  • Bradley JE, et al. (2009) Roles and regulation of Thy-1, a context-dependent modulator of cell phenotype. Biofactors. 35(3): 258-65.
  • Kisselbach L, et al. (2009) CD90 Expression on human primary cells and elimination of contaminating fibroblasts from cell cultures. Cytotechnology. 59(1): 31-44.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

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Mouse MAb

Rabbit PAb

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