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CALML3 抗體, 鼠單抗

  • CALML3 Antibody, Mouse MAb
  • CALML3 Antibody, Mouse MAb, Immunofluorescence
 CALML3 抗體產品資訊
免疫原:Recombinant Human CALML3 Protein (Catalog#12480-H07E)
Clone ID:03
Ig類型:Mouse IgG1
緩衝液:0.2 μm filtered solution in PBS
抗體製備方法:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CALML3 (rh CALML3; Catalog#12480-H07E; P27482; Met1-Lys149). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
 CALML3 Antibody IF Application Image
CALML3 Antibody, Mouse MAb, Immunofluorescence
Immunofluorescence staining of Human CALML3 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human CALML3 monoclonal antibody (15 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green). Positive staining was localized to cytoplasm and nucleus.
 CALML3 Antibody FC Application Image
CALML3 Antibody, Mouse MAb
Flow cytometric analysis of Human CALML3 expression on A431 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human CALML3, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Other CALML3 Antibody Products
CALML3 研究背景

Calmodulin-like protein 3 (CALML3) is similar to that of authentic calmodulin and may actually compete with calmodulin by binding, with different affinity, to cellular substrates. Calmodulin-like protein 3 (CALML3) is a tumor-sensitive protein specifically expressed in normal epithelial cells but downregulated in tumorigenesis. Downregulation of the protein is an early event in breast cancer development. One of the most pressing questions raised by the discovery of CLP/CALML3 is that of its potential targets. Although it is 85% identical to human calmodulin, the distinct properties of CLP suggest that it has specific targets or targets that only partially overlap with those of calmodulin. Research has identified the unconventional myosin-10 (Myo10) as a specific target of CALML3. The discovery of Myo10 as a specific target of CALML3 is highly significant and suggests multiple lines of further research such as investigations of the Ca2+ regulation of Myo10 and the role of the loss of CLP in epithelial differentiation, adhesion, and cancer. Cells expressing CALML3 displayed a striking increase in the number and length of myosin-10-positive filopodia and showed increased mobility in a wound healing assay.

 CALML3 參考資料
  • Bennett RD, et al. (2007) Calmodulin-like protein increases filopodia-dependent cell motility via up-regulation of myosin-10. J Biol Chem. 282(5): 3205-12.
  • Perochon A, et al. (2011) Calmodulin and calmodulin-like proteins in plant calcium signaling. Biochimie. 93(12): 2048-53.
  • Chinpongpanich A, et al. (2011) Biophysical characterization of calmodulin and calmodulin-like proteins from rice, Oryza sativa L. Acta Biochim Biophys Sin (Shanghai). 43(11): 867-76.
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    貨號: 12480-MM03-50
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    Datasheet & Documentation

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