Anti-Bcl-2 Antibody

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Anti-Bcl-2 Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-Bcl-2 Antibody
Validated applications
ELISA,ELISA(Det)
Species reactivity
Reacts with: Human
Specificity
Human Bcl-2
Immunogen
Recombinant Human BCL2 / Bcl-2 Protein (Catalog#10195-H08E)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human BCL2 / Bcl-2 (rh BCL2 / Bcl-2; Catalog#10195-H08E; P10415; Met1-Asp211). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Clone #04
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Bcl-2 Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution
ELISA 1:1000-1:2000
ELISA(Det) 1:1000-1:10000
Notes
ELISA(Det): This antibody will detect Human BCL2 / Bcl-2 in ELISA pair set (Catalog: # SEK10195). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10195-MM01T).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Bcl-2 Antibody: Alternative Names

Anti-Bcl-2 Antibody; Anti-PPP1R50 Antibody

Bcl-2 Background Information

BCL2 (B-cell leukemia/lymphoma 2, N-Histidine-tagged), also known as Bcl-2, belongs to the Bcl-2 family. Bcl-2 family proteins regulate and contribute to programmed cell death or apoptosis. It is a large protein family and all members contain at least one of four BH (bcl-2 homology) domains. Certain members such as Bcl-2, Bcl-xl and Mcl1 are anti-apoptotic, whilst others are pro-apoptotic. Most Bcl-2 family members contain a C-terminal transmembrane domain that functions to target these proteins to the outer mitochondrial and other intracellular membranes. It is expressed in a variety of tissues. BCL2 blocks the apoptotic death of some cells such as lymphocytes. It also regulates cell death by controlling the mitochondrial membrane permeability and inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
Full Name
B-cell CLL/lymphoma 2
References
  • Tsujimoto Y, et al. (1984) Cloning of the chromosome breakpoint of neoplastic B cells with the t(14;18) chromosome translocation. Science. 226(4678):1097-99.
  • Cleary ML, et al. (1986) Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation. Cell. 47(1):19-28.
  • Otake Y, et al. (2007) Overexpression of nucleolin in chronic lymphocytic leukemia cells induces stabilization of Bcl-2 / Bcl-2 mRNA. Blood. 109(7):3069-75.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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