Anti-Troponin C Antibody

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Anti-Troponin C Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-Troponin C Antibody
Validated applications
ELISA,IHC-P,IP
Species reactivity
Reacts with: Human, Cynomolgus
Specificity
Human Troponin C
Immunogen
Recombinant Human Troponin C/TNNC1 Protein (Catalog#11011-HNAE)
Preparation
Produced in rabbits immunized with purified, recombinant Human Troponin C/TNNC1 (rh Troponin C/TNNC1; Catalog#11011-HNAE; NP_003271.1; Met1-Glu161). Troponin C/TNNC1 specific IgG was purified by Human Troponin C/TNNC1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Troponin C Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
ELISA 1:5000-1:10000
IHC-P 1:500-1:2000
IP IP:5-10 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Troponin C Antibody (Rabbit Polyclonal antibody) Images

Immunochemical staining TNNC1 in human skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining TNNC1 in cynomolgus heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.

TNNC1 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

4 µL anti-TNNC1 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-TNNC1 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 18 kDa

Observed band size :16 kDa

Anti-Troponin C Antibody: Alternative Names

Anti-CMD1Z Antibody; Anti-CMH13 Antibody; Anti-TN-C Antibody; Anti-TNC Antibody; Anti-TNNC Antibody

Troponin C Background Information

Troponin I, also known as TNNC1, is part of the troponin complex. This complex contains 3 subunits: troponin I (TnI), troponin T (TnT) and troponin C (TnC). Troponin I is the inhibitory subunit, blocking actin-myosin interactions and thereby mediating striated muscle relaxation. It binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place. Because of it myosin cannot bind actin in relaxed muscle. When calcium binds to the Troponin C it causes conformational changes which lead to dislocation of troponin I and finally tropomyosin leaves the binding site for myosin on actin leading to contraction of muscle.
Full Name
troponin C type 1 (slow)
References
  • Kalaji FR. et al., 2012, Saudi J Kidney Dis Transpl. 23 (5): 939-45.
  • Wijnker PJ. et al., 2013, Am J Physiol Heart Circ Physiol. 304 (2): H260-8.
  • Solaro RJ. et al., 2013, Circ Res. 112 (2): 355-66.

Standard Antibody Development Service

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Mouse MAb

Rabbit PAb

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