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AARSD1 抗體, 兔單抗

Human AARSD1 抗體產品資訊
免疫原:Recombinant Human AARSD1 Protein (Catalog#14280-H07E)
Clone ID:012
Ig類型:Rabbit IgG
緩衝液:0.2 μm filtered solution in PBS
抗體製備方法:This antibody was obtained from a rabbit immunized with purified, recombinant Human AARSD1 (Catalog #14280-H07E; Q9BTE6-2; Thr176-Glu525).
Human AARSD1 抗體使用指導
特異性:Human AARSD1

WB: 5-10 μg/ml

ELISA: 0.1-0.2 μg/ml

This antibody can be used at 0.1-0.2 μg/ml with the appropriate secondary reagents to detect Human AARSD1. The detection limit for Human AARSD1 is approximately 0.0049 ng/well.

ICC/IF: 10-25 μg/mL

儲存方法:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human AARSD1 Antibody WB Application Image
Human AARSD1 Antibody IHC Application Image
AARSD1 Antibody, Rabbit MAb
Immunofluorescence staining of Human AARSD1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human AARSD1 monoclonal antibody (15 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Other AARSD1 Antibody Products
AARSD1 研究背景

AARSD1 belongs to the class-II aminoacyl-tRNA synthetase family, Alax-L subfamily. AARSD1 binds 1 zinc ion per subunit functions in trans to edit the amino acid moiety from incorrectly charged tRNA(Ala). Four transcript variants have been described for AARSD1: NM_025267.3, NM_001136042.2, NM_001142653.1 and NM_001142654.1. It has been determined that the latter two variants represent a distinct upstream locus, which is now represented by GeneID:100885848 (PTGES3L), while the former two variants represent readthrough transcripts between PTGES3L and this locus (AARSD1). The readthrough locus (PTGES3L-AARSD1) is now represented by GeneID:100885850.

Human AARSD1 參考資料
  • Tatham MH, et al. (2011) Comparative proteomic analysis identifies a role for SUMO in protein quality control. Oncogene. 19(17):2120-8.
  • Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell. 44(2):325-40.
  • Udeshi ND, et al. (2012) Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition. Mol Cell Proteomics. 11(5):148-59.
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    貨號: 14280-R012-50
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