Anti-4EBP1 Antibody (PE)

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Anti-4EBP1 Antibody (PE) (Mouse Monoclonal antibody) General Information

Product name
Anti-4EBP1 Antibody (PE)
Validated applications
FCM
Species reactivity
Reacts with: Human
Specificity
Human 4EBP1
Immunogen
Recombinant Human 4E-BP1 / EIF4EBP1 protein (Catalog#10022-H07E)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 4E-BP1 / EIF4EBP1 (rh 4E-BP1 / EIF4EBP1; Catalog#10022-H07E; NP_004086.1; Ser2-Ile118) and conjugated with PE under optimum conditions, the unreacted PE was removed.
Source
Monoclonal Mouse IgG1 Clone #04
Purification
Protein A
Formulation
Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Conjugate
PE
Concentration
2 μl/Test, 0.1 mg/ml
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Anti-4EBP1 Antibody (PE) (Mouse Monoclonal antibody) Images

Profile of anti-4EBP1 reactivity on HepG2 cells analyzed by flow cytometry.

Anti-4EBP1 Antibody (PE): Alternative Names

Anti-4E-BP1 Antibody; Anti-4EBP1 Antibody; Anti-BP-1 Antibody; Anti-PHAS-I Antibody

4EBP1 Background Information

The translational suppressor eIF4E binding protein-1, 4E-BP1 functions as a key regulator in cellular growth, differentiation, apoptosis and survival. The Eif4ebp1 gene, encoding 4E-BP1, is a direct target of a transcription factor activating transcription factor-4 (ATF4), a master regulator of gene expression in stress responses. 4E-BP1 is characterized by its capacity to bind specifically to eIF4E and inhibit its interaction with eIF4G. Phosphorylation of 4E-BP1 regulates eIF4E availability, and therefore, cap-dependent translation, in cell stress. Binding of eIF4E to eIF4G is inhibited in a competitive manner by 4E-BP1. Phosphorylation of 4E-BP1 decreases the affinity of this protein for eIF4E, thus favouring the binding of eIF4G and enhancing translation. 4E-BP1 is important for beta-cell survival under endoplasmic reticulum (ER) stress. 4E-BP1 mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. Recently, 4E-BP1 was found to be a key factor, which converges several oncogenic signals, phosphorylates the molecules, and drives the downstream proliferative signals. Recent studies showed that high expression of phosphorylated 4E-BP-1 (p-4E-BP1) is associated with poor prognosis, tumor progression, or nodal metastasis in different human cancers.
Full Name
eukaryotic translation initiation factor 4E binding protein 1
References
  • Azar R, et al. (2008) Phosphatidylinositol 3-kinase-dependent transcriptional silencing of the translational repressor 4E-BP1. Cell Mol Life Sci. 65(19): 3110-7.
  • Tominaga R, et al. (2010) The JNK pathway modulates expression and phosphorylation of 4E-BP1 in MIN6 pancreatic beta-cells under oxidative stress conditions. Cell Biochem Funct. 28(5): 387-93.
  • Ayuso MI, et al. (2010) New hierarchical phosphorylation pathway of the translational repressor eIF4E-binding protein 1 (4E-BP1) in ischemia-reperfusion stress. J Biol Chem. 285(45): 34355-63.

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