Anti-RPS3 Antibody

Cat: 101346-T36

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Anti-RPS3 Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-RPS3 Antibody
Validated applications
WB,IP
Species reactivity
Reacts with: Human
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Specificity
Human RPS3
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human 40S ribosomal protein S3/RPS3
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human 40S ribosomal protein S3/RPS3, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Anti-RPS3 Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution Notes
WB 1:500-1:2000  
IP 0.5-2 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-RPS3 Antibody (Rabbit Polyclonal antibody) Images

Human RPS3 Western blot (WB) 6589

Anti-Hexokinase I rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:27 kDa

Observed band size:32 kDa

Human RPS3 Immunoprecipitation(IP) 17415

RPS3 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

Lane B:0.5 mg Jurkat Whole Cell Lysate

2 µL anti-RPS3 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-RPS3 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 33 kDa

Observed band size: 33 kDa

Anti-RPS3 Antibody Alternative Names

Anti-S3 Antibody

RPS3 Background Information

Full Name
ribosomal protein S3
Product Description Host Clonality Application Catalog# (PDF)
Anti-RPS3 Antibody Rabbit Polyclonal WB,ICC/IF,IF,IP 101346-T38
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