( We provide with CD244 qPCR primers for gene expression analysis, HP100135 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人 2B4 / CD244 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||HG10042-ACG|
|人 2B4 / CD244 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||HG10042-ACR|
|人 2B4 / CD244 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||HG10042-CF|
|人 2B4 / CD244 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||HG10042-CH|
|人 2B4 / CD244 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||HG10042-CM|
|人 2B4 / CD244 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||HG10042-CY|
|人 2B4 / CD244 基因全長cDNA ORF(克隆載體)||HG10042-M|
|人 2B4 / CD244 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||HG10042-NF|
|人 2B4 / CD244 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||HG10042-NH|
|人 2B4 / CD244 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||HG10042-NM|
|人 2B4 / CD244 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||HG10042-NY|
|人 2B4 / CD244 基因全長cDNA ORF克隆 (表達載體)||HG10042-UT|
The CD244 antigen, also known as 2B4, is a cell surface glycoprotein implicated in the regulation of natural killer and T lymphocyte function. 2B4 is a member of the signaling lymphocyte activation molecule (SLAM)-related receptor family and is important for stimulating NK cell cytotoxicity and cytokine production, which is expressed on all NK cells, a subpopulation of T cells, monocytes and basophils. The 2B4 antigen identified on NK cells and T cells is capable of transmitting stimulatory signals and non-MHC-restricted killing. Reported as an activating receptor, human 2B4 can effectively activate and enhance NK cell–mediated cytotoxicity, induce secretion of IFN-γ and matrix metalloproteinases (MMPs), as well as NK cell invasiveness. As a cell surface glycoprotein of the Ig-superfamily structurally related to CD2-like molecules such as CD2, CD48, CD58, CD84, Ly-9, and SLAM, 2B4 (CD244) is expressed on all human NK cells, a subpopulation of T cells, basophils and monocytes. 2B4 activates NK cell mediated cytotoxicity, induces secretion of IFN-gamma and matrix metalloproteinases, and NK cell invasiveness.
Immune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: IP Antibodies Immune Checkpoint Detection: FCM Antibodies
Immune Checkpoint Proteins
Immune Checkpoint Targets Co-inhibitory Immune Checkpoint Targets