Anti-14-3-3 sigma Antibody

Cat: 10838-RP01
Contact us:
+86-400-890-9989
order@sinobiological.com
Distributors
All 14-3-3 sigma Reagents
CRO Services
Product recommended

All 14-3-3 sigma Reagents
Anti-14-3-3 sigma Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-14-3-3 sigma Antibody
Validated applications
ELISA
Species reactivity
Reacts with: Human
Specificity
Human 14-3-3 sigma
Immunogen
Recombinant Human 14-3-3 sigma / Stratifin / YWHAS / SFN protein (Catalog#10838-HNCE)
Preparation
Produced in rabbits immunized with purified, recombinant Human 14-3-3 sigma / Stratifin / YWHAS / SFN (rh 14-3-3 sigma / Stratifin / YWHAS / SFN; Catalog#10838-HNCE; NP_006133.1; Met 1-Ser 248). Total IgG was purified by Protein A affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-14-3-3 sigma Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-14-3-3 sigma Antibody Alternative Names
Anti-YWHAS Antibody
14-3-3 sigma Background Information

14-3-3 protein sigma (YWHAS), also known as stratifin (SFN) and epithelial cell marker protein 1, is a member of the14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2, and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally, YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.

Full Name
stratifin
References
  • Zakut-Houri R., et al.,(1985), Human p53 cellular tumor antigen: cDNA sequence and expression in COS cells. EMBO J. 4:1251-1255.
  • Lamb P., et al., (1986), Characterization of the human p53 gene.Mol. Cell. Biol. 6:1379-1385.
  • Harlow E., et al.,(1985), Molecular cloning and in vitro expression of a cDNA clone for human cellular tumor antigen p53.Mol. Cell. Biol. 5:1601-1610.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-14-3-3 sigma Antibody Rabbit Polyclonal ELISA 50576-RP01
    Anti-14-3-3 sigma Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 50576-RP02
    Anti-14-3-3 sigma Antibody Rabbit Polyclonal WB,ELISA,ICC/IF,IF,IP 10838-T54
    Anti-14-3-3 sigma Antibody Mouse Monoclonal FCM 10838-MM02
    Anti-14-3-3 sigma Antibody (PE) Mouse Monoclonal FCM 10838-MM02-P
    Anti-14-3-3 sigma Antibody Rabbit Polyclonal ELISA 10838-RP02
    info info
    添加购物车成功! 添加购物车失败!