Western Blot / WB FAQ

Questions & Solutionss

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Western blotting / WB Antibody
Western blotting / WB Antibody Features
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- Western blot introduction
What is Western Blot
Western Blot for Diagnosis
Western Blot Applications for Research
- Western Blot / WB tips
Sinobiological Western blot general tips
Western Blotting: 10 Technical Tips for Success
- Western Blot / WB FAQ
1. Why are there multiple bands on my blot?
2. Why is there a weak signal or no signal at all?
3. Why is there high background and/or diminished signal with anti-phosphor tyrosine antibodies?
4. Why does my antibody activity diminish in Western Blot over time?
5. How do I overcome inadequate or poor transfer problems?
6. How do I avoid "splotchy" or uneven Western Blots?
7. What can I use as positive control lysates for sinobiological WB antibodies?
8. Too many bands on a Western blot
9. No Signal or Weak Signal
10. Nonspecific Bands
11. High Background
12. How much protein should I analyse?
13. What percentage acrylamide gel should I use to resolve the proteins?
14. What membrane should I use?
15. What blocking buffer should I use?
16. What dilution of primary antibody should I use?
- Western Blot / WB protocol
Far Western Blot
ECL Western Blot
HRP Western Blot
Wet Western Blot
Semi-dry Western Blot
Densitometry Western Blot
Fluorescent Western Blot
Chemiluminescence Western Blot
Membrane Protein Western Blot
Western Blot Buffer / Reagents
Western Blot Sample Preparation
Western Blot Gel Electrophoresis
Western Blot Transfer
Western Blot Blocking
Western Blot Antibody Incubation
Western Blot Detection
- Western Blot / WB trouble shooting
High Background
Speckled Background
Multiple Bands
No Bands
Low Signal
Other Problems