Text Size:AAA
留言    收藏

V5 Synthetic Peptide

    產品資料評論相關產品實驗方法
    Product Introduction
     

    V5 peptide can be used to competitively elute V5-tagged fusion proteins from immobilized anti-V5 magnetic beads. Use V5 peptide to elute V5-tagged proteins. Elution yields will increase by incubating the peptide on the tube for 10-15 minutes at 30-37 oC for each elution fraction.

    Product Image
     
    V5 Synthetic Peptide
    Elution protocol
     

    1. Prepare V5 peptide at 1mg/mL in PBS.
    2. Add 50 μL 1 mg/mL V5 peptide to the protein combined beads (initially adding 50 μL Sino Biological V5 tag magnetic beads, TB100378), gently vortex to mix and incubate the sample at 37 oC on a rotator for 5-10 minutes. Elution can be performed at reduced temperatures, but lower yields result.
    3. Separate the beads on a magnetic separator and save the supernatant containing the target antigen.
    4. Repeat elution step once for higher recovery.

    Related Products
     
    Immunoprecipitation Kit -V5 Tag Immunomagnetic Beads TB100378
    Size / Price
    貨號: PP100378-5
    目錄價: 
    單價:      (You Save: )

    Datasheet & Documentation

    Contact Us
      您曾流覽過的產品
      All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
      請注意:所有產品都是“僅用於科研,而不能用於診斷或治療用途”