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V5 Synthetic Peptide

    Product Introduction

    V5 peptide can be used to competitively elute V5-tagged fusion proteins from immobilized anti-V5 magnetic beads. Use V5 peptide to elute V5-tagged proteins. Elution yields will increase by incubating the peptide on the tube for 10-15 minutes at 30-37 oC for each elution fraction.

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    V5 Synthetic Peptide
    Elution protocol

    1. Prepare V5 peptide at 1mg/mL in PBS.
    2. Add 50 μL 1 mg/mL V5 peptide to the protein combined beads (initially adding 50 μL Sino Biological V5 tag magnetic beads, TB100378), gently vortex to mix and incubate the sample at 37 oC on a rotator for 5-10 minutes. Elution can be performed at reduced temperatures, but lower yields result.
    3. Separate the beads on a magnetic separator and save the supernatant containing the target antigen.
    4. Repeat elution step once for higher recovery.

    Related Products
    Immunoprecipitation Kit -V5 Tag Immunomagnetic Beads TB100378
    Size / Price
    貨號: PP100378-5
    單價:      (You Save: )

    Datasheet & Documentation

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      All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.