V5 peptide can be used to competitively elute V5-tagged fusion proteins from immobilized anti-V5 magnetic beads. Use V5 peptide to elute V5-tagged proteins. Elution yields will increase by incubating the peptide on the tube for 10-15 minutes at 30-37 oC for each elution fraction.
1. Prepare V5 peptide at 1mg/mL in PBS.
2. Add 50 μL 1 mg/mL V5 peptide to the protein combined beads (initially adding 50 μL Sino Biological V5 tag magnetic beads, TB100378), gently vortex to mix and incubate the sample at 37 oC on a rotator for 5-10 minutes. Elution can be performed at reduced temperatures, but lower yields result.
3. Separate the beads on a magnetic separator and save the supernatant containing the target antigen.
4. Repeat elution step once for higher recovery.
-V5 Tag Immunomagnetic Beads