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Thoroughly Validated IHC Antibodies

The Importance of Antibody Reproducibility in Scientific Research

According to the statistics of "Nature", more than 70% of researchers have tried but failed to reproduce another scientist's experiments, and more than half have failed to reproduce their own experiments. One of the major factors contributing to the lack of reproducibility is the reagents used. Especially, the antibody reagents have an important role in the repeatability of publication data.

All of Sino Biological antibodies are developed and manufactured independently, and all the validation results are in the open. In order to provide customers more reliable antibodies, we have invested a lot of time and money in antibody quality verification and have established an highly strict QC process and various analytical methods.

Thoroughly Validated IHC Antibodies

The overall antibody validation steps in Sino Biological are shown as follows by taking the example of IHC antibodies.

Thoroughly Validated
Antigen Design Multiple Experimental
Controls
Antigen Blocking Multiple Tissues Batch Stability

Antigen Design

Produced in House

• 500+ professional team
• Ten years of high-quality reagent R&D experience
• A large inventory of 6,000+ recombinant proteins
• Five recombinant protein production platforms

90% by Eukaryotic Cells

• Closer to natural structure
• Better bio-activity
• Lower endotoxin: <0.1 EU / μg by LAL
• High purity: >95% by SDS-PAGE

Multiple Experimental Controls

CD3 antibody
A: Blank control B: Negative control C: Positive control

A: Blank control: There is no primary antibody in order to ensure that the secondary antibody does not non-specifically bind to certain cellular compartments.
B: Negative control: The protein you're interested is not present in the tissue. Therefore, if you see staining in this type of control, it indicates that the staining is non-specific.
C: Positive control: This is a tissue known to express the protein you are detecting. A negative result from the positive control demonstrates that the procedure has gone wrong.

Antigen Blocking

 

Antigen blocking means a method on the basis of pre-incubation of primary antibody with the immunogen to confirm the specificity of antibody. If the tissue shows no staining with antigen blocking, it is a powerful evidence that the antibody is specific to the target you're interested. But there are special cases. For instance, when the immunogen is the whole protein, the mixture of antibody plus protein may result in higher non-specific staining, but the mechanism is unclear now.

Multiple Tissues

Multiple Tissue Samples
CD3E    
  Tonsil sample 1   Tonsil sample 2   Tonsil sample 3
PSA    
  Prostate sample 1   Prostate sample 2   Prostate sample 3
 
Multiple Tissue Types
CD3E    
Tonsil   Hypognathadenitis   Lymph cancer
     
  Thymoma   Prostate    

Your target protein may express in different tissues, while usually the staining results of an antibody in different tissues are not exactly the same. Can you detect the target protein in the tissue you want with the antibody? This directly affects your experimental results. The test tissue provided by the antibody supplier may not be the same as the sample you want to study. Therefore, when the antibodies are validated in more tissue types and samples by the supplier, you can refer to more information and your experiments are more likely to be successful.

Batch Stability

Strict lot-to-lot validation to ensure the reproducibility
CD3E    
  Batch 1   Batch 2   Batch 3
CD147    
  Batch 1   Batch 2   Batch 3
 
Validation of Antibody Stability
CD3E    
Newly produced   After one year   After two years

Due to the inevitable differences existing in the animals immunized, different batches of polyclonal antibodies may not perform exactly the same in IHC staining. Although monoclonal antibodies are secreted by the same B lymphocytes, the changes in cell culture conditions and antibody purification methods may result in the changes in antibody properties. Therefore, whether it is the polyclonal antibody or the monoclonal antibody, the stability of antibodies between batches is of great importance.

請注意:所有產品都是“僅用於科研,而不能用於診斷或治療用途”