IL-15 Protein, Human, Recombinant: Product Information
> 95 % as determined by SDS-PAGE.
< 1 EU per μg of the protein as determined by the LAL method
1. Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The EC50 for this effect is typically 0.8-4 ng/mL. 2. Labeled biotin to IL-15 Protein, Human, Recombinant by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of IL-15 Protein, Human, Recombinant, Biotinylated (Cat. 10360-HNCE) bound to Anti-IL15 Antibody was 0.1nM.
A DNA sequence encoding the human IL15 (NP_000576.1) (Asn49-Ser162) was expressed.
The recombinant human IL15 consists of 114 amino acids and predicts a molecular mass of 12.8 kDa.
Lyophilized from sterile 50 mM Tris, 200 mM NaCl, pH 8.0. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
IL-15 Protein, Human, Recombinant: Images
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells.The EC50 for this effect is typically 0.8-4 ng/mL.
Labeled biotin to IL-15 Protein, Human, Recombinant by a certain molar ratio;
Using the Octet RED System, the affinity constant (Kd) of IL-15 Protein, Human, Recombinant, Biotinylated (Cat. 10360-HNCE) bound to Anti-IL15 Antibody was 0.1nM.
IL-15 Protein, Human, Recombinant: Alternative Names
IL-15 Protein, Human; Interleukin 15 Protein, Human
IL-15 Background Information
The protein encoded by IL15 gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively spliced transcript variants of this gene have been reported.
Mongini PKA, Gupta R, Boyle E, et al. TLR-9 and IL-15 synergy promotes the in vitro clonal expansion of chronic lymphocytic leukemia B cells. Journal of immunology (Baltimore, Md : 1950). 2015;195(3):901-923.
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