MMP12 Protein

MMP12 Protein Overview

MMP12 reagents

The matrix metalloproteases (MMPs) are a family of related matrix-degrading enzymes that are important in tissue remodeling and repair during development and inflammation. Abnormal expression is associated with various diseases such as tumor invasiveness, arthritis, and atherosclerosis. MMP activity may also be related to cigarette-induced pulmonary emphysema. Shapiro et al. (1993) cloned a cDNA for a metalloproteinase produced by human alveolar macrophages, which are known to have the capacity to degrade elastin (130160), by screening an alveolar macrophage cDNA library and a genomic library with the previously cloned mouse gene (Shapiro et al., 1992). The human gene, which they designated HME (human macrophage metalloelastase), produces a 1.8-kb transcript encoding a 470-amino acid protein that is 64% identical to the mouse protein. Both the mRNA and protein were detected in alveolar macrophages. As in the mouse, the predicted human 54-kD protein is processed by loss of both N- and C-terminal residues to a 22-kD mature form. The authors showed that recombinantly expressed HME was able to degrade insoluble elastin.

MMP12 protein family

Belongs to the peptidase M10A family.

MMP12 protein name

Recommended name
Macrophage metalloelastase
Short name
HME, macrophage elastase
Alternative name
Macrophage elastase Matrix metalloproteinase-12

MMP12 Protein Sequence

Species Human MMP12 protein
Length 470
Mass (Da) 54002
Sequence Human MMP12 protein sequence
Species Mouse MMP12 protein
Length 473
Mass (Da) 54971
Sequence Mouse MMP12 protein sequence
Species Rat MMP12 protein
Length 465
Mass (Da) 53738
Sequence Rat MMP12 protein sequence

MMP12 Protein Molecular Weight & PI

Macrophage metalloelastase precursor (EC (MME) (Macrophage elastase) (ME) (hME) (Matrix metalloproteinase-12) (MMP-12) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 106-470 Macrophage metalloelastase.

Molecular weight (Da)


Theoretical pI


MMP12 Protein Structure

Binary enzyme-product complexes of human MMP12
2003-03-19   Released:  2003-08-05
Deposition Author(s)
Bertini, I., Calderone, V., Fragai, M., Luchinat, C., Mangani, S., Terni, B.
Homo sapiens
Expression System
Escherichia coli
Experimental Data Snapshot
1.8500 Å
R-Value Free
R-Value Work
1OS9 From PDB

Human MMP12 protein Secondary structure

Recombinant MMP12 Protein Feature

MMP12 Protein, Human, Recombinant (catalytic domain)

High Purity
> 90 % as determined by SDS-PAGE
Low Endotoxin
Please contact us for more information.
High Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (AnaSpec, Catalog # 27076). The specific activity is > 800 pmoles/min/µg.

Bulk Order of Recombinant MMP12 Protein

Please Leave Us a Message if you have any questions regarding bulk price quote of our products on the website, our customer specialist will get back to you in 24 hours by email.

Custom Recombinant Protein Production Service Features

  • Over 10 years' experience for 6000+ recombinant proteins production.
  • One-stop service from gene synthesis and vector construction to protein expression and purification.
  • Multiple protein expression systems: bacterial, yeast, baculovirus-insect and mammalian expression system.
  • Multiple purification systems (30+) to choose.
  • High R & D ability with over 1000 new proteins per year and quick problem-solving ability.
  • Close to 100 bioreactors with various volume between 2-1000 L to guarantee high-throughput and large-scale production.

Custom Recombinant Protein Production Service Advantages

  • High-efficiency expression vectors
  • High cell density culturing
  • Proprietary medium formulation
  • >6000 proteins expression and purification experience
  • High-efficiency expression vectors
  • High cell density culturing

Recombinant Protein Production Service Description

We offer one-stop services for recombinant protein production using our advanced protein expression platform technologies. Our full services include protein gene synthesis, protein codon optimization, protein expression vector design, large scale cell culture and fermentation, protein purification, and protein quality control testing. We offer significant cost saving advantages and record speed in protein expression and bulk production. We also have extensive experiences and expertise in handling various types of recombinant protein purification projects, with or without purification tags.