MMP1 Protein

MMP1 Protein Overview

MMP1 reagents

Matrix metalloproteinases are zinc-dependent proteases that degrade extracellular matrix proteins. MMP1 is also known as collagenase (EC 3.4.23.7) (Nagase et al., 1992).

MMP1 protein family

Belongs to the peptidase M10A family.

MMP1 protein name

Recommended name
Interstitial collagenase
Short name
MMP-1
Aliases
interstitial collagenase
Alternative name
Fibroblast collagenase Matrix metalloproteinase-1 22 kDa interstitial collagenase 27 kDa interstitial collagenase

MMP1 Protein Sequence

Species Human MMP1 protein
Length 469
Mass (Da) 54007
Sequence Human MMP1 protein sequence
Species
Length
Mass (Da)
Sequence
Species Rat MMP1 protein
Length 15
Mass (Da) 1787
Sequence Rat MMP1 protein sequence

MMP1 Protein Molecular Weight & PI

Interstitial collagenase precursor (EC 3.4.24.7) (Fibroblast collagenase) (Matrix metalloproteinase-1) (MMP-1) [Contains: 22 kDa interstitial collagenase; 27 kDa interstitial collagenase] Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 100-469 Interstitial collagenase.

Molecular weight (Da)

42634.71

Theoretical pI

6.17

MMP1 Protein Structure

Crystal structure of human MMP1 catalytic domain at 2.2 A resolution
Deposited
2011-06-16   Released:  2011-09-21
Deposition Author(s)
Bertini, I., Calderone, V., Cerofolini, L., Fragai, M., Geraldes, C.F.G.C., Hermann, P., Luchinat, C., Parigi, G., Teixeira, J.
Organism(s)
Homo sapiens
Expression System
Escherichia coli
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.2000 Å
R-Value Free
0.278
R-Value Work
0.209
3SHI From PDB

Human MMP1 protein Secondary structure

MMP1 Protein Interaction

Recombinant MMP1 Protein Feature

MMP1 Protein, Human, Recombinant (His Tag)

High Purity
> 96 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
Measured by its ability to cleave the fluorogenic peptide substrate, McaPLGL-Dpa-AR-NH2, R&D System, Cat#ES010. The specific activity is >400 pmoles/min/μg (Activation description: The proenzyme needs to be activated by APMA for an activated form)

Recombinant MMP1 protein citations

Title
Upconversion fluorescence resonance energy transfer based biosensor for ultrasensitive detection of matrix metalloproteinase-2 in blood
Year
2012
Author
Y Wang, P Shen, C Li, Y Wang, Z Liu
Journal
Analytical chemistry
Title
3, 4‐Dinitrobenzamide Functionalized CdTe/ZnTe Quantum Dots as a Nanoprobe for Imaging Glutathione S‐Transferase in Living Cells
Year
2013
Author
Lu, J;Song, Y;Shi, W;Li, X;Ma - Chinese Journal of Chemistry, H;2013;
Journal
Chinese Journal of Chemistry
Title
Injectable Gel Scaffold Based on Biopolymer Microspheres via an Enzymatic Reaction
Year
2014
Author
Tan, H;Fan, M;Ma, Y;Qiu, J;Li, X;Yan, J;
Journal
Adv Healthc Mater
Title
Poly (m-phenylenediamine)-Based Fluorescent Nanoprobe for Ultrasensitive Detection of Matrix Metalloproteinase 2
Year
2014
Author
Z Wang, X Li, D Feng, L Li, W Shi, H Ma
Journal
Analytical chemistry
Title
Signal on fluorescence biosensor for MMP-2 based on FRET between semiconducting polymer dots and a metal organic framework
Year
2014
Author
Yang, W;Zhang, G;Weng, W;Qiu, B;Guo, L;Lin, Z;
Journal
RSC Advances

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