Anti-CXCL7 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-CXCL7 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-CXCL7 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-CXCL7 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-CXCL7 Magnetic Beads-IP Kit Product Description

The Anti-CXCL7 magnetic Beads, conjugated with Anti-CXCL7 antibody, are used for immuneprecipitation (IP) of CXCL7 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CXCL7 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CXCL7 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-CXCL7 Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-CXCL7 Antibody(50145-T52)
Immunogen
Recombinant Mouse NAP-2 / PPBP / CXCL7 protein (Catalog#50145-M07E)
Species Reactivity
Mouse NAP-2 / PPBP / CXCL7
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse NAP-2 / PPBP / CXCL7 (Catalog#50145-M07E; Q9EQI5; Ile49-Ile109). NAP-2 / PPBP / CXCL7 specific IgG was purified by Mouse NAP-2 / PPBP / CXCL7 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-CXCL7 Magnetic Beads Immunoprecipitation (IP) Kit: Alternative Names

Anti-2400003M24RikALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-AI854500ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-b-TG1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-beta-TGALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-CTAP3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-CTAPIIIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-Cxcl7ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-LA-PF4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-LDGFALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MDGFALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-NAP-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-NAP-2-L1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-Scyb7ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-TGBALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-TGB1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-THBGB1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

CXCL7 Background Information

Pro-platelet basic protein (PPBP) is also known as Chemokine (C-X-C motif) ligand 7 (CXCL7) and nucleosome assembly protein (Nap-2). Nap-2 / PPBP / CXCL7 is released in large amounts from platelets following their activation and is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. Nap-2 / PPBP / CXCL7 has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells. Nap-2 is a ligand for CXCR1 and CXCR2, and Nap-2, Nap-2 (73), Nap-2 (74), Nap-2 (1-66), and most potent Nap-2 (1-63) are chemoattractants and activators for neutrophils.
Full Name
pro-platelet basic protein (chemokine (C-X-C motif) ligand 7)
References
  • Walz A, et al. (1989) Effects of the neutrophil-activating peptide NAP-2, platelet basic protein, connective tissue-activating peptide III and platelet factor 4 on human neutrophils. J Exp Med. 170(5):1745-50.
  • Walz A, et al. (1990) Generation of the neutrophil-activating peptide NAP-2 from platelet basic protein or connective tissue-activating peptide III through monocyte proteases. J Exp Med. 171(2): 449-54.
  • Loetscher P, et al. (1994) Both interleukin-8 receptors independently mediate chemotaxis. Jurkat cells transfected with IL-8R1 or IL-8R2 migrate in response to IL-8, GRO alpha and NAP-2. FEBS Lett. 341(2-3): 187-92.
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