Anti-Mannan Binding Lectin / MBL2 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-Mannan Binding Lectin / MBL2 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-Mannan Binding Lectin / MBL2 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-Mannan Binding Lectin / MBL2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-Mannan Binding Lectin / MBL2 Magnetic Beads-IP Kit Product Description

The Anti-Mannan Binding Lectin / MBL2 magnetic Beads, conjugated with Anti-Mannan Binding Lectin / MBL2 antibody, are used for immuneprecipitation (IP) of Mannan Binding Lectin / MBL2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Mannan Binding Lectin / MBL2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Mannan Binding Lectin / MBL2 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-Mannan Binding Lectin / MBL2 Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-Mannan Binding Lectin / MBL2 Antibody(100438-T36)
Immunogen
A synthetic peptide corresponding to the C-terminus of the human MBL2
Species Reactivity
human MBL2
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with A synthetic peptide corresponding to the C-terminus of the human MBL2, and purified by antigen affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-Mannan Binding Lectin / MBL2 Magnetic Beads Immunoprecipitation (IP) Kit: Alternative Names

Anti-COLEC1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-HSMBPCALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MBLALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MBL2DALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MBPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MBP-CALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MBP1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MBPDALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

Mannan Binding Lectin / MBL2 Background Information

MBL (mannose-binding lectin) is primarily a liver-derived collagen-like serum protein, which binds sugar structures on micro-organisms and on dying host cells and is one of the four known mediators that initiate activation of the complement system via the lectin pathway. MBL and the ficolins (Ficolin-1, Ficolin-2 and Ficolin-3) are soluble collagen-like proteins that are involved in innate immune defence. They bind sugar structures or acetylated compounds present on microorganisms and on dying host cells and they initiate activation of the lectin complement pathway in varying degrees. MBL2 encodes the mannose-binding lectin, which is a key player in the innate immune system and has recently been found to play a role in development of type 1 diabetes and gestational diabetes mellitus. Common variant alleles situated both in promoter and structural regions of the MBL2 gene influence the stability and the serum concentration of the protein. Several polymorphisms in the promoter and structural regions of MBL2 adversely affect the plasma concentration and oligomeric state of MBL. The possession of mutant alleles has been linked to disease outcome for a variety of bacterial and viral infections. Mutant MBL2 haplotypes have been linked to disease progression and response to therapy in HCV infection.
Full Name
mannose-binding lectin (protein C) 2, soluble
References
  • Garred P, et al. (2006) Mannose-binding lectin and its genetic variants. Genes Immun. 7(2): 85-94.
  • Brown KS, et al. (2007) Mannan binding lectin and viral hepatitis. Immunol Lett. 108(1): 34-44.
  • Garred P. (2008) Mannose-binding lectin genetics: from A to Z. Biochem Soc Trans. 36(Pt 6): 1461-6.
  • Garred P, et al. (2009) MBL2, FCN1, FCN2 and FCN3-The genes behind the initiation of the lectin pathway of complement. Mol Immunol. 46(14): 2737-44.
  • Muller YL, et al. (2010) Functional Variants in MBL2 Are Associated With Type 2 Diabetes and Pre-Diabetes Traits in Pima Indians and the Old Order Amish. Diabetes. 59(8): 2080-5.
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