Anti-ERK2 Magnetic Beads Immunoprecipitation (IP) Kit

Price:
Size:
Number:

Anti-ERK2 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-ERK2 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-ERK2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-ERK2 Magnetic Beads-IP Kit Product Description

The Anti-ERK2 magnetic Beads, conjugated with Anti-ERK2 antibody, are used for immuneprecipitation (IP) of ERK2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing ERK2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound ERK2 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-ERK2 Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-ERK2 Antibody(10030-T52)
Immunogen
Recombinant Human ERK2/MAPK1/MAPK2 Protein (Catalog#10030-H09B)
Species Reactivity
Human ERK2/MAPK1/MAPK2
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human ERK2/MAPK1/MAPK2 (rh ERK2/MAPK1/MAPK2; Catalog#10030-H09B; NP_002736.3; Met1-Ser360). ERK2/MAPK1/MAPK2 specific IgG was purified by Human ERK2/MAPK1/MAPK2 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-ERK2 Magnetic Beads Immunoprecipitation (IP) Kit: Alternative Names

Anti-ERKALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-ERK-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-ERK2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-ERT1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MAPK2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-p38ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-p40ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-p41ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-p41mapkALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-p42-MAPKALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-P42MAPKALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PRKM1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PRKM2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

ERK2 Background Information

MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. ERK is a versatile protein kinase that regulates many cellular functions. Growing evidence suggests that extracellular signal-regulated protein kinase 1/2 (ERK1/2) plays a crucial role in promoting cell death in a variety of neuronal systems, including neurodegenerative diseases. It is believed that the magnitude and the duration of ERK1/2 activity determine its cellular function. Activation of ERK1/2 are implicated in the pathophysiology of spinal cord injury (SCI). ERK2 signaling is a novel target associated with the deleterious consequences of spinal injury. ERK-2, also known as Mitogen-activated protein kinase 1 (MAPK1), is a member of the protein kinase superfamily and MAP kinase subfamily. MKP-3 is a dual specificity phosphatase exclusively specific to MAPK1 for its substrate recognition and dephosphorylating activity. The activation of MAPK1 requires its phosphorylation by upstream kinases. Upon activation, MAPK1 translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. MAPK1 is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. MAPK1 acts as a transcriptional repressor which represses the expression of interferon gamma-induced genes. Transcriptional activity is independent of kinase activity. The nuclear-cytoplasmic distribution of ERK2 is regulated in response to various stimuli and changes in cell context. Furthermore, the nuclear flux of ERK2 occurs by several energy- and carrier-dependent and -independent mechanisms. ERK2 has been shown to translocate into and out of the nucleus by facilitated diffusion through the nuclear pore, interacting directly with proteins within the nuclear pore complex, as well as by karyopherin-mediated transport. ERK2 interacts with the PDE4 catalytic unit by binding to a KIM (kinase interaction motif) docking site located on an exposed beta-hairpin loop and an FQF (Phe-Gln-Phe) specificity site located on an exposed alpha-helix. These flank a site that allows phosphorylation by ERK, the functional outcome of which is orchestrated by the N-terminal UCR1/2 (upstream conserved region 1 and 2) modules.
Full Name
mitogen-activated protein kinase 1
Research Areas
References
  • Houslay MD, et al. (2003) The role of ERK2 docking and phosphorylation of PDE4 cAMP phosphodiesterase isoforms in mediating cross-talk between the cAMP and ERK signalling pathways. Biochem Soc Trans. 31(Pt 6): 1186-90.
  • Jivan A, et al. (2010) Reconstitution of the Nuclear Transport of the MAP Kinase ERK2. Methods Mol Biol. 661: 273-85.
  • Yu CG, et al. (2010) Involvement of ERK2 in traumatic spinal cord injury. J Neurochem. 113(1): 131-42.
  • Subramaniam S, et al. (2010) ERK and cell death: ERK1/2 in neuronal death. FEBS J. 277(1): 22-9.
Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait