DR4 / TRAIL R1 Matched ELISA Antibody Pair Set,Human

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DR4 / TRAIL R1 Matched ELISA Antibody Pair Set,Human General Information

Product name
DR4 / TRAIL R1 Matched ELISA Antibody Pair Set,Human
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Human DR4 / TRAIL R1
Assay range
2.34-150 pg/mL
Materials provided
1. Capture Antibody 0.5 mg/mL of mouse anti-Human TRAIL R1 / CD261 / TNFRSF10A monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10408-MM01)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human TRAIL R1 / CD261 / TNFRSF10A monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10408-R001)
3. Standard Each vial contains 30 ng of recombinant Human TRAIL R1 / CD261 / TNFRSF10A. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 150 pg/mL is recommended.
Product overview
This DR4 / TRAIL R1 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human DR4 / TRAIL R1 . It contains Human DR4 / TRAIL R1 capture antibody, Human DR4 / TRAIL R1 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human DR4 / TRAIL R1 protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

DR4 / TRAIL R1 Matched ELISA Antibody Pair Set,Human Images

DR4 / TRAIL R1 Matched ELISA Antibody Pair Set,Human: Alternative Names

APO2 Matched ELISA Antibody Pair Set, Human; CD261 Matched ELISA Antibody Pair Set, Human; DR4 Matched ELISA Antibody Pair Set, Human; MGC9365 Matched ELISA Antibody Pair Set, Human; TNFRSF10A Matched ELISA Antibody Pair Set, Human; TRAILR-1 Matched ELISA Antibody Pair Set, Human; TRAILR1 Matched ELISA Antibody Pair Set, Human

DR4 / TRAIL R1 Background Information

Tumor necrosis factor receptor superfamily, member 1a (TRAIL R1), also known as TRAIL receptor 1 (TRAIL R1) or CD261 antigen, is a member of the TNF-receptor superfamily. This receptor is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF1/TRAIL), and thus transduces cell death signal and induces cell apoptosis. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. TRAIL R1/CD261/TNFRSF1A serves as a receptor for the cytotoxic ligand TNFSF1/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. TRAIL R1 can promote the activation of NF-kappa-B. TRAIL R1/CD261/TNFRSF1A induces apoptosis of many transformed cell lines but not of normal tissues, even though its death domain-containing receptor, DR4, is expressed on both cell types.
Full Name
tumor necrosis factor receptor superfamily member 10a
References
  • Kimberley FC, et al. (2005) Following a TRAIL: update on a ligand and its five receptors.". Cell Res. 14 (5): 359–72.
  • Schneider P, et al. (1997) Characterization of two receptors for TRAIL.". FEBS Lett. 416 (3): 329–34.
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