MAP1LC3A cDNA ORF Clone, Human, N-HA tag

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MAP1LC3A cDNA ORF Clone, Human, N-HA tag: General Information

Gene
Species
Human
NCBI Ref Seq
RefSeq ORF Size
408 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence.
Description
Full length Clone DNA of Human microtubule-associated protein 1 light chain 3 alpha with N terminal HA tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
Restriction Sites
KpnI + XbaI(6kb+0.41kb)
Tag Sequence
HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.

MAP1LC3A cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

MAP1LC3A cDNA ORF Clone, Human, N-HA tag: Validated Images

MAP1LC3A cDNA ORF Clone, Human, N-HA tag: Alternative Names

ATG8E cDNA ORF Clone, Human; LC3 cDNA ORF Clone, Human; LC3A cDNA ORF Clone, Human; MAP1ALC3 cDNA ORF Clone, Human; MAP1BLC3 cDNA ORF Clone, Human

MAP1LC3A Background Information

LC3A, also known as MAP1LC3A, is one of the light chain subunits that functions together with both MAP1A and/or MAP1B. MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. As a light chain subunit, MAP1LC3A has an important part in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP1LC3A is expressed as two alternatively spliced isoforms that are expressed in testis, brain, heart, liver and skeletal muscle, but are absent in thymus and peripheral blood leukocytes.
Full Name
microtubule-associated protein 1 light chain 3 alpha
References
  • He H., et al.,(2003), Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B. J. Biol. Chem. 278:29278-29287.
  • Deloukas P., et al., (2001), The DNA sequence and comparative analysis of human chromosome 20.Nature 414:865-871.
  • Bechtel S., et al.,(2007), The full-ORF clone resource of the German cDNA consortium.BMC Genomics 8:399-399.
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