IL-13 ELISA Kit, Human

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IL-13 ELISA Kit, Human: General Information

Product name
IL-13 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Cell supernatant
Sensitivity
0.59 pg/mL
Assay range
4.69-300 pg/mL
Specificity
Recognizes both recombinant and natural Human IL-13
Interference
Preparations of the following factors at 10 ng/mL in a mid-range recombinant Human IL13 control were assayed for interference. No significant interference was observed.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 50.00 101.00 208.00
SD 2.23 2.32 7.53
CV(%) 4.4% 2.3% 3.6%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 50.00 102.00 206.00
SD 2.89 1.15 5.86
CV(%) 5.6% 1.1% 2.8%
Recovery
The recovery of Human IL13 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
84
Range
81 -87%
Sample
supernatant (n=3)
Average % Recovery
84
Range
77 -94%
Sample
urine (n=3)
Average % Recovery
84
Range
81 -87%
Linearity
Supernatant
  Recovery of detected
1:2 108%
1:4 104%
1:8 108%
1:16 118%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This IL-13 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-13 protein in Cell supernatant . It contains recombinant Human IL-13, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

IL-13 ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human IL13. The factors listed below were prepared at 10 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

IL-13 ELISA Kit, Human: Alternative Names

ALRH ELISA Kit, Human; IL-13 ELISA Kit, Human; P600 ELISA Kit, Human

IL-13 Background Information

Interleukin 13 (IL-13) is a single-chain glycosylated polypeptide, which belongs to the IL-13/IL-4 family. IL-13 protein is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL-13 exerts its effects through a multi-subunit receptor comprising the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains (IL-13 Rα1 and IL-13 Rα2). As a cytokine, IL-13 protein is critical in regulating inflammatory, immune responses and diseases. In addition, it inhibits the production of pro-inflammatory cytokines and chemokines, and thus down-regulates macrophage activity. IL-13 protein and antibody is more importantly implicated as a central mediator of immunoregulatory processes in various cell types.
Full Name
interleukin 13
References
  • Junttila IS, et al. (2008) Tuning sensitivity to IL-4 and IL-13: differential expression of IL-4Ralpha, IL-13Ralpha1, and gammac regulates relative cytokine sensitivity. J Exp Med. 205(11): 2595-608.
  • Shimamura T,et al. (2008) Novel role of IL-13 in fibrosis induced by nonalcoholic steatohepatitis and its amelioration by IL-13R-directed cytotoxin in a rat model. J Immunol. 181(7): 4656-65.
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