DC-SIGN ELISA Kit, Human

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DC-SIGN ELISA Kit, Human: General Information

Product name
DC-SIGN ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
11.8 pg/mL
Assay range
31.25-2000 pg/mL
Specificity
Recognizes both recombinant and natural Human DC-SIGN
Interference
Preparations of ICAM2、ICAM3、CLU、ICAM5 and LSP1 at 20 ng/mL in a mid-range recombinant human CD209 control were assayed for interference. No significant interference was observed.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.45 0.84 1.56
SD 0.02 0.04 0.06
CV(%) 3.9% 4.5% 3.9%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.36 0.78 1.45
SD 0.03 0.07 0.11
CV(%) 7.5% 8.8% 7.8%
Recovery
The recovery of Human CD209 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
110.53802101782999
Range
109%-114%
Linearity
Serum
  Recovery of detected
1:2 101%
1:4 99%
1:8 73%
1:16 84%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This DC-SIGN ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human DC-SIGN protein in Serum . It contains recombinant Human DC-SIGN, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

DC-SIGN ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural human CD209. The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.A sample containing 50 ng/mL of recombinant human CLEC4M reads as 0.7ng/mL(1.4% cross-reactivity).
Preparations of ICAM2、ICAM3、CLU、ICAM5 and LSP1 at 20 ng/mL in a mid-range recombinant human CD209 control were assayed for interference. No significant interference was observed.

DC-SIGN ELISA Kit, Human: Alternative Names

CD209 ELISA Kit, Human; CDSIGN ELISA Kit, Human; CLEC4L ELISA Kit, Human; DC-SIGN ELISA Kit, Human; DC-SIGN1 ELISA Kit, Human; MGC129965 ELISA Kit, Human

DC-SIGN Background Information

Dendritic cell (DC)-specific intercellular adhesion molecule 3 (ICAM-3) grabbing nonintegrin (DC-SIGN), also known as CD29, is a type II transmembrane protein on DCs with a C-type lectin extracellular domain, is capable of binding ICAM-3 on resting T cells in the secondary lymphoid organs, providing the initial contact between these cells during the establishment of cell-mediated immunity. It is not only a pattern recognition receptor but implicated in immunoregulation of DCs. It has important role in mediating DC adhesion, migration, inflammation, activating primary T cell, triggering immune response and participating in immune escape of pathogens and tumors. DC-SIGN also mediates capture and internalization of viral, bacterial, and fungal pathogens by dendritic cells, such as HIV-1, Ebola virus, cytomegalovirus, Dengue virus, and hepatitis C virus. DC-SIGN is unique in that it regulates adhesion processes, such as DC trafficking and T-cell synapse formation, as well as antigen capture. Moreover, even though several C-type lectins have been shown to bind HIV-1, DC-SIGN does not only capture HIV-1 but also protects it in early endosomes allowing HIV-1 transport by DC to lymphoid tissues, where it enhances trans infection of T cells.
Full Name
CD209 molecule
References
  • Geijtenbeek TB, et al. (2002) DC-SIGN, a C-type lectin on dendritic cells that unveils many aspects of dendritic cell biology. J Leukoc Biol. 71(6): 921-31.
  • Masso M. (2003) DC-SIGN points the way to a novel mechanism for HIV-1 transmission. MedGenMed. 5(2): 2.
  • Zhou T, et al. (2006) DC-SIGN and immunoregulation. Cell Mol Immunol. 3(4): 279-83.
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