CD31 ELISA Kit, Human

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CD31 ELISA Kit, Human: General Information

Product name
CD31 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum,Urine,Saliva
Sensitivity
38.43 pg/mL
Assay range
218.75-14000 pg/mL
Specificity
Recognizes both recombinant and natural Human CD31
Interference
Preparations of the following factors at 200 ng/mL in a mid-range recombinant Human CD31 control were assayed for interference. No significant interference was observed.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 2,726.00 5,525.00 9,960.00
SD 73.51 207.52 294.48
CV(%) 2.7% 3.8% 3.0%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 2,812.00 5,655.00 11,422.00
SD 127.05 394.09 1,280.75
CV(%) 4.5% 7.0% 11.2%
Recovery
The recovery of Human PECAM1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
84
Range
81 -87%
Sample
urine (n=3)
Average % Recovery
84
Range
81 -87%
Linearity
Serum
  Recovery of detected
1:2 93%
1:4 107%
1:8 98%
1:16 90%
Supernatant
  Recovery of detected
1:2 93%
1:4 98%
1:8 109%
1:16 95%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This CD31 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CD31 protein in Serum,Urine,Saliva . It contains recombinant Human CD31, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

CD31 ELISA Kit, Human: Images

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human CD31. The factors listed below were prepared at 200 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

CD31 ELISA Kit, Human: Alternative Names

CD31 ELISA Kit, Human; CD31/EndoCAM ELISA Kit, Human; endoCAM ELISA Kit, Human; GPIIA' ELISA Kit, Human; PECA1 ELISA Kit, Human; PECAM-1 ELISA Kit, Human

CD31 Background Information

The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 13-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.
Full Name
platelet/endothelial cell adhesion molecule 1
References
  • Deaglio S, et al. (2000) CD38/CD31, a receptor/ligand system ruling adhesion and signaling in human leukocytes. Chem Immunol. 75: 99-120.
  • Kohler S, et al. (2009) Life after the thymus: CD31+ and CD31- human naive CD4+ T-cell subsets. Blood. 113(4): 769-74.
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