Human CD3 (humanized OKT3) Antibody-Human IgG4 (GMP-grade)

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Human CD3 (humanized OKT3) Antibody-Human IgG4 (GMP-grade) ( antibody) General Information

Product name
Human CD3 (humanized OKT3) Antibody-Human IgG4 (GMP-grade)
Validated applications
FCM,Func/A
Species reactivity
Reacts with: Human
Specificity
Human CD3 epsilon / CD3e
Preparation
Human CD3 (humanized OKT3) Antibody is a humanized antibody of Orthoclone OKT3 (GMP-grade procution). Orthoclone OKT3 is an immunosuppressant drug given to reduce acute rejection in patients with organ transplants. It is a monoclonal antibody targeted at the CD3 receptor, a membrane protein on the surface of T cells. It was the first monoclonal antibody to be approved for clinical use in humans.
Source
Human IgG4 Clone #H001
Formulation
0.2 μm filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH6.0
Conjugate
Unconjugated
Endotoxin
< 0.5 EU/mg
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Human CD3 (humanized OKT3) Antibody-Human IgG4 (GMP-grade) ( antibody) Validated Applications

Application Dilution
FCM 0.5-2 μg/Test
Notes
Agonist Activity (Func/A): Agonist Activity – Measured by its ability to induce proliferation of human T cells. The ED50 for this effect is typically 2-10 ug/mL.
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Human CD3 (humanized OKT3) Antibody-Human IgG4 (GMP-grade) ( antibody) Images

The staining effect of Human CD3 (humanized OKT3) antibody to CD3 of Human Peripheral Blood Lymphocytes by Flow cytometric analysis.
A: Lymphocytes were stained with PE-conjugated human CD3 (humanized OKT3) antibody.
B: The binding effect of PE-conjugated humanized OKT3 antibody(Red histograms) in the lymphocytes can be blocked by pre-incubation of the CD3 antibody (Miltenyi Biotec, 130-093-387) for 20min (blue histograms).
CD3+ T cell staining of lymphocytes using Human CD3 (humanized OKT3) antibody by Flow cytometric analysis.
A: Lymphocytes were stained with both PE-conjugated CD3(humanized OKT3) antibody and OKT3-FITC-eBioscience (eBioscience, 11-0037-41).
B: Lymphocytes were stained with PE-conjugated humanized OKT3 antibody and CD19-APC (BD, 555415).
Effect of Human CD3 (humanized OKT3) antibody on the proliferation and IFN-gamma secretion of CD4+ T cell.
The isolated CD4+ T cells(1e5/well) were cultured for 4 days in the 96-well cell plates coated with serial dilutions of CD3 (humanized OKT3) antibody.
A. The cell proliferation level was detected using MTT detection;
B. IFN-gamma secretion in the medium was measured by IFN-gamma Quantitative ELISA Kit.

Human CD3 (humanized OKT3) Antibody-Human IgG4 (GMP-grade): Alternative Names

Anti-CD3 epsilon Antibody; Anti-IMD18 Antibody; Anti-T3E Antibody; Anti-TCRE Antibody

CD3 epsilon / CD3e Background Information

T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature naïve T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.
Full Name
CD3e molecule, epsilon (CD3-TCR complex)
References
  • Fischer A, et al. (2005) CD3 deficiencies. Curr Opin Allergy Clin Immunol. 5(6): 491-5.
  • Wang Y, et al. (2009) A conserved CXXC motif in CD3epsilon is critical for T cell development and TCR signaling. PLoS Biol. 7(12): e1000253.
  • Martnez-Martn N, et al. (2009) Cooperativity between T cell receptor complexes revealed by conformational mutants of CD3epsilon. Sci Signal. 2(83): ra43.
  • Deford-Watts LM, et al. (2009) The cytoplasmic tail of the T cell receptor CD3 epsilon subunit contains a phospholipid-binding motif that regulates T cell functions. J Immunol. 183(2): 1055-64.

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