HRG / HPRG Matched ELISA Antibody Pair Set,Human

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HRG / HPRG Matched ELISA Antibody Pair Set,Human General Information

Product name
HRG / HPRG Matched ELISA Antibody Pair Set,Human
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Human HRG / HPRG
Assay range
15.63-1000 pg/mL
Materials provided
1. Capture Antibody 1 mg/mL of mouse anti-Human HRG/HPRG monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10836-MM01)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human HRG/HPRG polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.4 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 35 ng of recombinant Human HRG/HPRG. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.
Product overview
This HRG / HPRG Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human HRG / HPRG . It contains Human HRG / HPRG capture antibody, Human HRG / HPRG detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human HRG / HPRG protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

HRG / HPRG Matched ELISA Antibody Pair Set,Human Images

HRG / HPRG Matched ELISA Antibody Pair Set,Human: Alternative Names

HPRG Matched ELISA Antibody Pair Set, Human; HRGP Matched ELISA Antibody Pair Set, Human; THPH11 Matched ELISA Antibody Pair Set, Human

HRG / HPRG Background Information

Histidine-rich glycoprotein, also known as HRG and HPRG, is a glycoprotein located in plasma and platelets, and contains an unusually large amount of histidine and proline. In human, five distinct domains are recognized in the mature HPRG molecule. There are two N-terminal cystatin-like modules (aa 19 - 254) and one His-Pro-rich region (aa 35 - 497) that is flanked by two Pro-rich segments (aa 276 - 321 and 498 - 525). The His-Pro-rich region contains 1 tandem repeats with an HHPHG motif, and the N- and C-termini are linked by a disulfide bond. The specific functions of HRG remain unclear, but it is known that the protein binds heme, dyes and divalent metal ions. It inhibits rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. HPRG is evolutionarily, functionally and structurally related to cleaved high molecular weight kininogen (HKa), an anti-angiogenic polypeptide that stimulates apoptosis of proliferating endothelial cells through binding to cell-surface tropomyosin. The antiangiogenic activity of the multidomain plasma protein HPRG is localized to its histidine-proline-rich (H/P) domain and has recently been shown to be mediated, at least partially, through binding to cell-surface tropomyosin in fibroblast growth factor-2-activated endothelial cells.
Full Name
histidine-rich glycoprotein
References
  • Guan X, et al. (2004) Histidine-proline rich glycoprotein (HPRG) binds and transduces anti-angiogenic signals through cell surface tropomyosin on endothelial cells. Thromb Haemost. 92(2): 403-12.
  • Doate F, et al. (2004) Peptides derived from the histidine-proline domain of the histidine-proline-rich glycoprotein bind to tropomyosin and have antiangiogenic and antitumor activities. Cancer Res. 64(16): 5812-7.
  • From Diatoms to Malaria: Synthesis and Application of Functional Materials
    Author
    Swartz, JD;
    Year
    2011
    Journal
    Thesis
  • The nuclear bile acid receptor FXR controls the liver derived tumor suppressor histidine-rich glycoprotein
    Author
    Deuschle, U;Birkel, M;Hambruch, E;Hornberger, M;Kinzel, O;Perović-Ottstadt, S;Schulz, A;Hahn, U;Burnet, M;Kremoser, C;
    Year
    2014
    Journal
    Int. J. Cancer
    Application
    ELISA (plasma sample)
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