Her2 / ERBB2 Protein, Cynomolgus, Recombinant (ECD, Fc Tag)

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Her2 / ERBB2 Protein, Cynomolgus, Recombinant (ECD, Fc Tag): Product Information

Purity
> 85 % as determined by SDS-PAGE.
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Activity
1.Measured by its binding ability in a functional ELISA.
2.Immobilized cynomolgus ErbB2 (Cat:90295-C02H) at 10μg/mL (100μL/well) can bind herceptin, the EC50 of herceptin is 30-100ng/mL.
Protein Construction
A DNA sequence encoding the cynomolgus ERBB2 (Met1-Thr652) [ Rhesus ERBB2 (XP_001090430.1,Asp121Asn,Leu122Pro); similar to Macaca fascicularis ERBB2 (EHH58073.1)], was expressed with the Fc region of human IgG1 at the C-terminus.
Expressed Host
HEK293 Cells
Species
Cynomolgus
Predicted N Terminal
Thr 23
Molecule Mass
The recombinant cynomolgus ERBB2 consists 871 amino acids and predicts a molecular mass of 96.4 kDa.
Formulation
Lyophilized from sterile PBS, pH 7.4.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Her2 / ERBB2 Protein, Cynomolgus, Recombinant (ECD, Fc Tag): Images

Her2 / ERBB2 Background Information

Epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD34, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.
Full Name
erb-b2 receptor tyrosine kinase 2
References
  • Krawczyk N, et al. (2009) HER2 status on persistent disseminated tumor cells after adjuvant therapy may differ from initial HER2 status on primary tumor. Anticancer Res. 29(10): 4019-24.
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