|All Antibody Services||All Protein Services||All Gene Services||All Biological Services|
|Full services:||From gene cloning/synthesis to purified proteins
|Host Cells:||Choices of Expression Hosts:
Transient Transfection in HEK293 Cells
Transient Transfection in CHO cells
Stable expression in CHO cells
Yeast cell expression system
Insect cell/baculovirus expression system
E. coli. microbial expression system
|HA Tag Design:||HA-tag (YPYDVPDYA-tag) at N- or C-terminus
|Cleavage Site:||Option to design a cleavage site between the tag and protein
Selection of Cleavage Protease Enzyme: Enterokinase (EK),
HRV-3C (human rhinovirus protease), TEV
|Purification:||HA (YPYDVPDYA) tag specific antibody conjugated affinity clumn
HA-tag Affinity Purification Resin
|Deliverable:||Purified recombinant proteins (quantities to be specified by customer)|
|Quality Specification:||typically >90% purity by SDS page
|Typical Timeline:||8-12 weeks|
What is an HA-tag?
An HA-tag is derived from the human influenza hemagglutinin (HA) molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. The HA-tag does not appear to interfere with the bioactivity or the biodistribution of the recombinant HA-tagged protein.
What is the HA-tag Peptide's Nucleotide and Amino Acid Sequence?
The HA tag's nucleotide sequence is: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3'
The HA tag's amino acid sequence is: YPYDVPDYA
What is the Molecular Wieght of the HA-tag?
The molecular weight of the HA-tag (YPYDVPDYA-tag) peptide is 1102.15 Da.
Why use an HA-tag for protein expression and production?
Adding an HA-tag to a protein's C- or N-terminus can facilitate
HA-tag (YPYDVPDYA-tag) has been widely used in recombinant protein expression and production. The HA-tag allows simple and efficient affinity purification of the tagged protein using HA-tag specific antibody conjugated to agarose-beads. The HA-tag (YPYDVPDYA-tag) also can be used for detection in western blot by using a HA-tag-specific antibody. HA-tag can be added to either C- or N-terminus of a protein for expression in virtually all expression systems.
Sino Biological has successfully produced many proteins with the HA-tag and purified with HA-tag affinity chromatography. We are highly experienced in designing the HA-tag according to the protein structure and handle any problems arising from protein purification process.
Sino Biological has also developed highly sensitive HA-tag (YPYDVPDYA -tag) specific antibodies for ...... < Learn More>